MABC86
Anti-Nesprin-2 Antibody, clone K20-478
clone K20-478, from mouse
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Synonym(s):
Nesprin-2, Nuclear envelope spectrin repeat protein 2, Nucleus and actin connecting element protein, Protein NUANCE, Synaptic nuclear envelope protein 2, Syne-2
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
K20-478, monoclonal
species reactivity
human, mouse
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... SYNE2(23224)
General description
Nesprin-2 (SYNE-2) is also known as Nuclear envelope spectrin repeat protein 2 or Nucleus and actin connecting element protein, and it functions to network organelles and the actin cytoskeleton in order maintain subcellular spatial organization. Nesprin-2 connects nuclei to the cytoskeleton by interacting with the nuclear envelope and cytoplasmic F-actin, and is required for centrosome migration to the apical cell surface during early ciliogenesis. Nesprin-2 is widely expressed, with high levels seen in kidney, adult and fetal liver, stomach and placenta. Defects in SYNE2 are responsible for Emery-Dreifuss muscular dystrophy type 5 (EDMD5).
Immunogen
His-tagged recombinant protein corresponding to human Nesprin-2.
Application
Detect Nesprin-2 using this mouse monoclonal antibody, Anti-Nesprin-2 Antibody, clone K20-478 validated for use in western blotting, ICC & IP.
Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected Nesprin-2 in HepG2 and COS-7 cells.
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Nesprin-2 in HA-MKS3 transfected HEK293 whole cell extracts. Immunoprecipitated sample was then subjected to Western Blotting using the same representative lot of antibody (Dawe, H. R., et al. (2009). J Cell Sci. 122(Pt 15): 2716-2626.).
Western Blotting Analysis: A representative lot from an independent laboratory detected Nesperin-2 in IMCDE whole cell extracts. High levels of a 25 kDa ABD-containing isoform was observed only at post-confluence (+72 hours); however, a second major ABD-containing isoform was observed at 55 kDa before and after confluence (Dawe, H. R., et al. (2009). J Cell Sci. 122(Pt 15): 2716-2626.).
Dylight® is a registered trademark of Thermo Fisher Scientific. Alexa Fluor is a registered trademark of Molecular Probes, Inc.
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Nesprin-2 in HA-MKS3 transfected HEK293 whole cell extracts. Immunoprecipitated sample was then subjected to Western Blotting using the same representative lot of antibody (Dawe, H. R., et al. (2009). J Cell Sci. 122(Pt 15): 2716-2626.).
Western Blotting Analysis: A representative lot from an independent laboratory detected Nesperin-2 in IMCDE whole cell extracts. High levels of a 25 kDa ABD-containing isoform was observed only at post-confluence (+72 hours); however, a second major ABD-containing isoform was observed at 55 kDa before and after confluence (Dawe, H. R., et al. (2009). J Cell Sci. 122(Pt 15): 2716-2626.).
Dylight® is a registered trademark of Thermo Fisher Scientific. Alexa Fluor is a registered trademark of Molecular Probes, Inc.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
Quality
Evaluated by Western Blotting in COS-7 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Nesprin-2 in 10 µg of COS-7 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Nesprin-2 in 10 µg of COS-7 cell lysate.
Target description
~250 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
DyLight is a registered trademark of Pierce Biotechnology, Inc.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Aravindh Subramani et al.
Cells, 12(13) (2023-07-14)
The spread of tumor cells and the formation of distant metastasis remain the main causes of mortality in cancer patients. However, the mechanisms governing the release of cells from micro-environmental constraints remain unclear. E-cadherin negatively controls the invasion of epithelial
Yasunao Kamikawa et al.
Cell death discovery, 7(1), 152-152 (2021-07-07)
The nuclear envelope (NE) safeguards the genome and is pivotal for regulating genome activity as the structural scaffold of higher-order chromatin organization. NE had been thought as the stable during the interphase of cell cycle. However, recent studies have revealed
Maria Chiara Lionetti et al.
Biophysical journal, 118(9), 2319-2332 (2020-04-23)
The nuclear morphology of eukaryotic cells is determined by the interplay between the lamina forming the nuclear skeleton, the chromatin inside the nucleus, and the coupling with the cytoskeleton. Nuclear alterations are often associated with pathological conditions as in Hutchinson-Gilford
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