MABC605
Anti-TXNIP Antibody, clone 3A7.1
clone 3A7.1, from mouse
Sign Into View Organizational & Contract Pricing
All Photos(5)
Synonym(s):
Thioredoxin-interacting protein, Thioredoxin-binding protein 2, Vitamin D3 up-regulated protein 1
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
3A7.1, monoclonal
species reactivity
mouse, human
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... TXNIP(10628)
General description
Thioredoxin-interacting protein (UniProt Q9H3M7; also known as Thioredoxin-binding protein 2, Vitamin D3 up-regulated protein 1) is encoded by the TXNIP (also known as VDUP1) gene (Gene ID 10628) in human. Thioredoxin interacting protein (TXNIP) binds thioredoxin active cysteine residue and suppresses its antioxidant activity. TXNIP overexpression results in increased cellular ROS level and oxidative cell death, while TXNIP suppression enhances cellular resistance to oxidative injury. TXNIP is also shown to inhibit cellular glucose uptake by downregulating glucose transporter 1 (GLUT1) plasma membrane localization, and TXNIP expression is found to be markedly suppressed in many cancers, making TXNIP a potential anticancer therapeutic target.
Specificity
Clone 3A7.1 targets an epitope within the N-terminal half of TXNIP. Cross-reactivity to human and mouse spliced isoform 2 is likely, but has not been confirmed.
Immunogen
GST-tagged recombinant fragment from the N-terminal half of human TXNIP.
Application
Anti-TXNIP Antibody, clone 3A7.1 is an antibody against TXNIP for use in Western Blotting, Immunocytochemistry.
Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected TXNIP in HUVECs, A431, HeLa, and NIH/3T3 cells.
Quality
Evaluated by Western Blotting in K562 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected TXNIP in 10 µg of K562 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected TXNIP in 10 µg of K562 cell lysate.
Target description
~44 kDa observed. 43.66/37.32 kDa (human isoform 1/2) and 44.36/44.29 kDa (mouse isoform 1/2) calculated.
Physical form
Format: Purified
Other Notes
Concentration: Please refer to lot specific datasheet.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service