MABC32-AF488
Anti-p62 (Sequestosome-1) Antibody, clone 11C9.2 Antibody, Alexa Fluor™ 488 Conjugate
from mouse, ALEXA FLUOR™ 488
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Synonym(s):
EBI3-associated protein of 60 kDa, EBIAP, p60, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
mouse
Quality Level
conjugate
ALEXA FLUOR™ 488
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
monoclonal
species reactivity
mouse, human, rat
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
isotype
IgM
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... SQSTM1(8878)
Related Categories
General description
Sequestosome-1 (UniProt: Q13501; also known as EBI3-associated protein of 60 kDa; EBIAP; p60; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62) is encoded by the SQSTM1 (also known as ORCA, OSIL) gene (Gene ID 8878) in human. Sequestosome-1 is a multifunctional protein that binds ubiquitin and regulates the activation of the nuclear factor kappa-B (NF-kappaB) signaling pathway. It functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 (TRAF6) to mediate the activation of NF-kappaB in response to upstream signals. Defects in sequestosome-1 are a cause of Paget disease of bone (PDB), a metabolic bone disease affecting the axial skeleton and is characterized by focal areas of increased and disorganized bone turn-over due to activated osteoclasts. Mutations in SQSTM1 gene can lead to frontotemporal dementia and/or amyotrophic lateral sclerosis 3. Frontotemporal dementia is characterized by frontal and temporal lobe atrophy associated with neuronal loss, gliosis, and dementia. Amyotrophic lateral sclerosis is characterized by the death of motor neurons in the brain, brainstem, and spinal cord, resulting in fatal paralysis.
Specificity
Clone 11C9.2, Alexa Fluor™ 488 Conjugate detects p62 in HeLa cells.
Immunogen
GST-tagged recombinant fragment of 200 amino acids from the internal region of human p62 (Sequestosome-1).
Application
Detect p62 using this mouse monoclonal Anti-p62 (Sequestosome-1) antibody, clone 11C9.2, Alexa Fluor™ 488 Conjugate, Cat. No. MABC32-AF488, validated for use in Flow Cytometry and Immunocytochemistry.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected p62 Sequestosome-1 in A431 and NIH/3T3 cells.
Flow Cytometry Analysis: A 1:400 dilution from a representative lot detected p62 Sequestosome-1 in one million HeLa cells.
Flow Cytometry Analysis: A 1:400 dilution from a representative lot detected p62 Sequestosome-1 in one million HeLa cells.
Quality
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected p62 (Sequestosome-1) in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected p62 (Sequestosome-1) in HeLa cells.
Target description
47.7 kDa calculated.
Physical form
Purified mouse monoclonal antibody IgM in PBS with 1.5% BSA and 0.05% sodium azide.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
WGK
WGK 2
Regulatory Information
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