MABC272
Anti-Annexin VI Antibody, clone 4B11.1
clone 4B11.1, from mouse
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Annexin A6, 67 kDa calelectrin, Annexin VI, Annexin-6, Calphobindin-II, CPB-II, Chromobindin-20, Lipocortin VI, Protein III, p68, p70
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
4B11.1, monoclonal
species reactivity
mouse, human
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ANXA6(309)
General description
The protein Annexin A6 or alternatively known as 67 kDa calelectrin, Annexin VI, Annexin-6, Calphobindin-II (CPB-II), Chromobindin-20, Lipocortin VI, Protein III, p68, or p70 and encoded by the human gene named ANXA6 or ANX6. Annexin VI is part of a large family of Annexin proteins and shares with the family the ability to bind negatively charged phospholipids in a calcium dependent manner. Localized in the plasma membrane annexins are important in many cellular functions as the provide scaffolds and play roles in vesicle transport, exocytosis, endocytosis and calcium transport and conduction. Recent research indicates that Annexin VI can interact with PKCalpha and EGFR in A431 cells to help regulate the phosphorylation of pY-EGFR and the associated activation of downstream signaling events associated with cancer. Annexin VI in normal tissue is expressed most strongly in myocardial tissue where it associates with sarcomeric α-actinin and is part of the sarcomere structure. Annexin VI also regulates terminal chondrocyte differentiation by modulating key signaling pathway activities.
Immunogen
GST-tagged recombinant protein corresponding to human Annexin VI.
Application
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-Annexin VI Antibody, clone 4B11.1 is validated for use in western blotting & ICC for the detection of Annexin VI.
Western Blotting Analysis: 1 µg/mL from a representative lot detected Annexin VI in 10 µg of C2C12 cell lysate.
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Annexin VI in HeLa and A431 cells.
Alexa Fluor™ is a registered trademark of Life Technologies.
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Annexin VI in HeLa and A431 cells.
Alexa Fluor™ is a registered trademark of Life Technologies.
Quality
Evaluated by Western Blotting in SH-SY5Y cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Annexin VI in 10 µg of SH-SY5Y cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Annexin VI in 10 µg of SH-SY5Y cell lysate.
Target description
~75 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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