Anti-MMP-11 (Stromelysin-3) Antibody, clone 5ST-4A9

Stromelysin-3 (UniProt: P24347; also known as SL-3, ST3, Matrix metalloproteinase-11, MMP-11) is encoded by the MMP11 (also known as STMY3) gene (Gene ID: 4320) in human. MMP-11 is shown to be differentially expressed in tumor tissue and plays a role in tumor invasion. It is shown to be well expressed in stromal cells of breast carcinomas. It has little expression in adult organs under normal physiological conditions. During development, it is expressed in many processes involved in cell migration and tissue morphogenesis. MMP-11 has multiple zinc and calcium binding sites. It binds 2 zinc ions per subunit and one calcium ion per subunit. MMP-11 contains 4 hemopexin domains (aa 291-339; 340-382; 384-432; and 433-480). It is synthesized with a signal peptide (aa 1-31) and a propeptide region (aa 32-97) that are cleaved to generate mature enzyme. It contains a conserved cysteine that is present in the cysteine-switch motif (aa 78-85) that binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion activates the enzyme. The precursor is cleaved by a furin endopeptidase. Expression levels of MMP-11 are a reliable indicator of greatest risk for cancer recurrence in breast carcinoma, pancreatic tumors, and colon cancer. (Ref.: Roscilli, G et al. (2014). J. Transl. Med. 12; 54).

Clone 5ST-4A9 detects MMP-11 (stromelysin 3) in human cells.

His-tagged full length recombinant human MMP-11 (Stromelysin-3).

Immunohistochemistry Analysis: A representative lot detected MMP-11 (Stromelysin-3) in Immunohistochemistry applications in (Ahmad, A., et. al. (1998) Am J Pathol. 152(3):721-8; Nakopoulou, L., et. al. (2002). Mod Pathol. 15(11):1154-61).

Western Blotting Analysis: A representative lot detected MMP-11 (Stromelysin-3) in Western Blotting applications in (von Marschall, Z., et. al. (1998) Gut. 43(5):692-8; Motrescu, E.R., et. al. (2008). Oncogene. 27(49):6347-55; Luo, D., et. al. (1999). J Biol Chem. 274(52):37177-85).

Immunohistochemistry Analysis: A representative lot detected MMP-11 (Stromelysin-3) in Immunohistochemistry applications in (Cribier, B., et. al. (2002). J Am Acad Dermatol. 46(3):408-13; von Marschall, Z., et. al. (1998) Gut. 43(5):692-8; Andarawewa, K.L., et. al. (2005). Cancer Res. 65(23):10862-71; Motrescu, E.R., et. al. (2008). Oncogene. 27(49):6347-55.

Immunoprecipitation Analysis: A representative lot detected MMP-11 (Stromelysin-3) in Immunoprecipitation applications in (Schonbeck, U., et. al. (1999). J Exp Med. 189(5):843-53).

Immunohistochemistry Analysis: A 1:50, 250, 1,000 dilution from a representative detected MMP-11 (Stromelysin-3) in human placenta and human breast cancer tissue.

Research Category
Apoptosis & Cancer

Evaluated by Western Blotting in PC-3 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected MMP-11 (Stromelysin-3) in 10 µg of PC-3 cell lysate.

~55 kDa observed; 54.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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