MABC14
Anti-VHL Antibody, clone 11E12
clone 11E12, from mouse
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Synonym(s):
von Hippel-Lindau disease tumor suppressor, Protein G7, elongin binding protein, von Hippel-Lindau syndrome, pVHL
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
11E12, monoclonal
species reactivity
human, mouse
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... VHL(7428)
General description
von Hippel-Lindau protein (VHL) is most widely recognized as belonging to an E3 ubiquitin ligase complex that specifically targets hypoxia-inducible factors (HIFs) for the purpose of polyubiquitination and proteolytic degradation. Within this complex, VHL acts as a target recruitment subunit for HIFα and its subsequent ubiquitination. The complex also contains proteins such as B and C elongins, Rbx1 and Cul2. Inactivation of VHL has been linked to renal cell carcinoma development, therefore suggesting that VHL plays a role as a tumor suppressor. VHL expression has been observed in both fetal and adult kidney and brain tissues. Defects in VHL expression are causal to renal cell carcinoma type 1 (RCC1), erythrocytosis familial type 2 (ECYT2), von Hippel-Lindau disease (VHLD), and pheochromocytoma.
Immunogen
Purified human VHL
Application
Immunoprecipitation: It has been shown that this VHL clone will immunoprecipitate VHL from HEK 293 cells (Shoenfeld, 1998).
Research Category
Apoptosis & Cancer
Signaling
Apoptosis & Cancer
Signaling
Research Sub Category
Tumor Markers
Ubiquitin & Ubiquitin Metabolism
Tumor Markers
Ubiquitin & Ubiquitin Metabolism
This Anti-VHL Antibody, clone 11E12 is validated for use in WB, IP for the detection of VHL.
Quality
Evaluated by Western Blot in mouse fetal brain tissue lysate.
Western Blot Analysis: 1 µg/mL of the antibody detected VHL in 10 µg of mouse fetal brain tissue lysate.
Western Blot Analysis: 1 µg/mL of the antibody detected VHL in 10 µg of mouse fetal brain tissue lysate.
Target description
~ 24 kDa observed MW. VHL SUMOylation may result in high MW bands (Cai, 2010).
Physical form
Format: Purified
Protein G
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse fetal brain tissue lysate
Mouse fetal brain tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Chenglai Fu et al.
Circulation research, 122(3), 457-472 (2017-12-28)
Inositol polyphosphate multikinase (IPMK) and its major product inositol pentakisphosphate (IP5) regulate a variety of cellular functions, but their role in vascular biology remains unexplored. We have investigated the role of IPMK in regulating angiogenesis. Deletion of IPMK in fibroblasts
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