Anti-MAGEA3 Antibody, clone 57B

Melanoma-associated antigen 3 (UniProt: P43357; also known as Antigen MZ2-D, Cancer/testis antigen 1.3, CT1.3, MAGE-3 antigen) is encoded by the MAGEA3 (also known as MAGE3) gene (Gene ID: 4102) in human. MAGE-3 is a member of the melanoma-associated antigen gene family of cancer antigens that are expressed in several neoplastic, but not normal, tissues with the exception of testes. In melanoma cells, it enhances viability and serves as an antigen for cytolytic T lymphocytes. MAGEA3 expression is not reported in kidney tumors, leukemias and lymphomas. MAGEA3 expression is normally limited to early developmental stages. It enhances ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. It is also reported to enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. It is proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex.

Clone 57B specifically targets a sequence in the N-terminal region of melanoma associated antigen 3 in human testes.

Epitope: domain of:

His-tagged recombinant human melanoma associated antigen 3 containing the polyhistidine tail.

Anti-MAGEA3 Antibody, clone 57B, Cat. No. MABC1150, detects multiple MAGE-A proteins. Validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Immunocytochemistry Analysis: A representative lot detected MAGEA3 in Immunocytochemistry applications. (Hofbauer, G.F., et. al. (1997) Am J Pathol. 151(6):1549-53; Landry, C., et. al. (2000). Int J Cancer. 86(6):835-41; Kocher, T., et. al. (1995). Cancer Res. 55(11):2236-9; Juretic, A., et. al. (2003). Lancet Oncol. 4(2):104-9).

Western Blotting Analysis: A representative lot detected MAGEA3 in Western Blot applications. (Kocher, T., et. al. (1995). Cancer Res. 55(11):2236-9; Rimoldi, D., et. al. (2000). Int J Cancer. 86(5):749-51; SHultz-Thater, E., et. al. (2011). Int J Cancer. 129(5):1137-48).

Immunohistochemistry Analysis: A representative lot detected MAGEA3 in Immunohistochemistry applications. (Hofbauer, G.F., et. al. (1997) Am J Pathol. 151(6):1549-53; Aubry, F., et. al. (2001). Cancer. 92(11):2778-85; Cheville, J.C., et. al. (1999). Mod Pathol. 12(10):974-8; Chitale D.A., et. al. (2005). Mod Pathol. 18(1):119-26; Groeper, C., et. al. (2007). Int J Cancer. 120(2):337-43; Juretic, A., et. al. (2003). Lancet Oncol. 4(2):104-9.

Research Category
Apoptosis & Cancer

Evaluated by Immunohistochemistry in human testis tissue.

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected MAGEA3 in human testis tissue.

34.75 kDa calculated.

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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