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MABC002F

Sigma-Aldrich

Mouse IgG1 Negative Control, clone Ci4, FITC conjugate

Mouse IgG1 Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.

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UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.42

biological source

mouse

Quality Level

100

conjugate

FITC conjugate

antibody form

purified immunoglobulin

clone

Ci4, monoclonal

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunofluorescence: suitable

isotype

IgG1

shipped in

wet ice

target post-translational modification

unmodified

Specificity

This antibody reacts with chicken immunoglobulin. No reaction with human cell surface or plasma components has been observed. However some binding to Fc receptors may occur.

Immunogen

Chicken immunoglobulin

Application

Fluorescein conjugated mouse IgG1 is a monoclonal antibody intended for use as a negative control in immunofluorescence and flow cytometry. Its use enables an estimation of non-specific binding of fluorescein conjugated mouse monoclonal antibodies to cell surface components in peripheral blood and tissue.

SUGGESTED USAGE

Flow cytometry and immunofluorescence - use 10 μl direct from the vial per 100 μl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) or bone marrow cells in 100 μl buffer.
Research Category
Secondary & Control Antibodies

Epitope Tags & General Use
Research Sub Category
Isotype Control Antibodies

Physical form

Purified immunoglobulin. Fluorescein conjugated, ready to use. The antibody is supplied in 1.0ml phosphate buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry.

Storage and Stability

Store at 2 to 8°C, for up to 6 months. DO NOT FREEZE. Protect from light.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Marc Elser et al.
American journal of cancer research, 13(10), 4597-4612 (2023-11-16)
Glioblastomas (GBM) are the most common primary brain tumors in adults and associated with poor clinical outcomes due to therapy resistances and destructive growth. Interactions of cancer cells with the extracellular matrix (ECM) play a pivotal role in therapy resistances

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