MAB8878
Anti-Cdk1 Antibody, clone A17.1.1
clone A17.1.1, Chemicon®, from mouse
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Synonym(s):
p34cdc2, CDC2
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
A17.1.1, monoclonal
species reactivity
human, Xenopus, mouse, rat, chicken
manufacturer/tradename
Chemicon®
technique(s)
activity assay: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Cdk1(12534)
Specificity
Recognizes a 34 kDa protein cdk1, identified as cdk1/p34cdc2 protein kinase (a catalytic subunit of Maturation Promoting Factor). Its epitope maps near the C-terminus of protein and its core is thought to be LGTPNNEV. It shows no cross-reaction with cdk2/p32 and other cyclin dependent kinases (cdks).
Immunogen
Raised against Xenopus p34cdc2.
Application
Anti-Cdk1 Antibody, clone A17.1.1 is a Mouse Monoclonal Antibody for detection of Cdk1 also known as p34cdc2, CDC2 & has been validated in IF, IP, EA, WB, IHC(P).
Frozen Sections
Immunofluorescence
Immunoprecipitation: (Native/denatured, Protein A; 2μg/mg protein lysate)
Immunohistology (Formalin, 2-4μg/mL for 30 min at RT)
Paraffin Sections (staining requires antigen retrieval).
Western Blotting
Kinase Assay
Optimal working dilutions must be determined by the end user.
p34cdc2, a serine/threonine kinase, is activated by cyclins, presumably by dephosphorylation of tyrosine residues. MAB8878 inhibits the activation of p34cdc2 kinase by cyclins and precipitates an active kinase.
Immunofluorescence
Immunoprecipitation: (Native/denatured, Protein A; 2μg/mg protein lysate)
Immunohistology (Formalin, 2-4μg/mL for 30 min at RT)
Paraffin Sections (staining requires antigen retrieval).
Western Blotting
Kinase Assay
Optimal working dilutions must be determined by the end user.
p34cdc2, a serine/threonine kinase, is activated by cyclins, presumably by dephosphorylation of tyrosine residues. MAB8878 inhibits the activation of p34cdc2 kinase by cyclins and precipitates an active kinase.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Physical form
Format: Purified
Purified immunoglobulin. 0.2 mg/mL in 10 mM PBS, pH 7.4, containing 0.2% BSA and 0.09% Sodium Azide.
Storage and Stability
Maintain at 2° to 8°C in undiluted aliquots for up to 12 months from date of receipt.
Analysis Note
Control
POSITIVE CONTROL:
HeLa or NIH3T3 cells. Germinal center in a tonsil or reactive lymph node. Cellular localization is predominantly nuclear, with some cytoplasmic staining.
POSITIVE CONTROL:
HeLa or NIH3T3 cells. Germinal center in a tonsil or reactive lymph node. Cellular localization is predominantly nuclear, with some cytoplasmic staining.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Ian M McDonald et al.
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During mammalian meiotic prophase I, programmed DNA double-strand breaks are repaired by non-crossover or crossover events, the latter predominantly occurring via the class I crossover pathway and requiring the cyclin N-terminal domain-containing 1(CNTD1) protein. Using an epitope-tagged Cntd1 allele, we
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