MAB8293
Anti-Parvovirus B19 Antibody, aa 328-344 of VP2 capsid protein, clone R92F6
clone R92F6, Chemicon®, from mouse
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UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
R92F6, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1
shipped in
wet ice
Specificity
Recognizes Human parvovirus B19. Recognizes an epitope common to VP1 and VP2 structural proteins of B19. In B19 infected tissues, MAB8293 staining is localized predominantly in the nucleus though cytoplasmic staining may also be observed.
Immunogen
Epitope: a.a. 328-344 of VP2 capsid protein
Native parvovirus B19 purified from human plasma.
Application
Anti-Parvovirus B19 Antibody, aa 328-344 of VP2 capsid protein, clone R92F6 is an antibody against Parvovirus B19 for use in ELISA, IF, IH(P) & WB.
EIA: 1:200.
IFA: 1:200-1:400.
Immunohistochemistry on formalin-fixed paraffin-embedded tissue sections: 1:50-1:100. High temperature citrate buffer antigen retrieval is highly recommended.
Western Blot: 1:1,000.
Final working dilutions must be determined by end user.
IFA: 1:200-1:400.
Immunohistochemistry on formalin-fixed paraffin-embedded tissue sections: 1:50-1:100. High temperature citrate buffer antigen retrieval is highly recommended.
Western Blot: 1:1,000.
Final working dilutions must be determined by end user.
Research Category
Infectious Diseases
Infectious Diseases
Research Sub Category
Infectious Diseases - Viral
Infectious Diseases - Viral
Physical form
Format: Purified
Liquid in 20mM Phosphate buffer, pH7.6, and 0.25M NaCl with 0.1% sodium azide as a preservative.
Protein A purified
Storage and Stability
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Parvovirus positive patient sample
Parvovirus positive patient sample
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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HepG2 hepatocellular carcinoma cells are a non-permissive system for B19 virus infection.
Bonvicini, F; Filippone, C; Manaresi, E; Zerbini, M; Musiani, M; Gallinella, G
The Journal of General Virology null
Neutralization of human parvovirus B19 by plasma and intravenous immunoglobulins.
Jens Modrof,Andreas Berting,Bjorn Tille,Andreas Klotz,Christina Forstner,Sandra Rieger et al.
Transfusion null
Roles of E4orf6 and VA I RNA in adenovirus-mediated stimulation of human parvovirus B19 DNA replication and structural gene expression.
Winter, K; von Kietzell, K; Heilbronn, R; Pozzuto, T; Fechner, H; Weger, S
Journal of virology null
Detection of parvovirus B19 capsid proteins in testicular tissues.
Monica E Polcz,Laura A Adamson,Reva S Datar,Larry J Fowler,Jacqueline A Hobbs
Urology null
Rhiannon R Penkert et al.
Vaccines, 9(8) (2021-08-29)
Children with sickle cell disease (SCD) suffer life-threatening transient aplastic crisis (TAC) when infected with parvovirus B19. In utero, infection of healthy fetuses may result in anemia, hydrops, and death. Unfortunately, although promising vaccine candidates exist, no product has yet
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