MAB8129
Anti-Cytomegalovirus Antibody, immediate early, clone 7D12.2
clone 7D12.2, Chemicon®, from mouse
Synonym(s):
CMV
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.42
biological source
mouse
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
7D12.2, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
suitability
not suitable for immunohistochemistry (Paraffin)
shipped in
wet ice
Specificity
Reacts with an immediate early protein M.W. 68 kD. Can detect CMV infection 1 hour post-infection exhibiting a nuclear and cytoplasmic staining which reaches peak intensity between 8-24 hours.
With CMV the antigens expressed at different times are listed as:
Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.
Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.
Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD
With CMV the antigens expressed at different times are listed as:
Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.
Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.
Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD
Immunogen
Epitope: immediate early
MRC-5 cells infected with CMV AD169.
Application
Immunohistochemistry.
Immunocytochemistry.
IFA at 1:3,000-1:6,000 on acetone fixed cells.
Does not work on paraffin embedded tissue sections.
Dilute with buffer pH 7.4-7.6 to desired working volume.
For extensive dilution, protein containing or other stabilizing medium should be used.
Final working dilutions must be determined by end user.
Immunocytochemistry.
IFA at 1:3,000-1:6,000 on acetone fixed cells.
Does not work on paraffin embedded tissue sections.
Dilute with buffer pH 7.4-7.6 to desired working volume.
For extensive dilution, protein containing or other stabilizing medium should be used.
Final working dilutions must be determined by end user.
Research Category
Infectious Diseases
Infectious Diseases
Research Sub Category
Infectious Diseases - Viral
Infectious Diseases - Viral
This Anti-Cytomegalovirus Antibody, immediate early, clone 7D12.2 is validated for use in IF, WB, IC, IH for the detection of Cytomegalovirus.
Physical form
Format: Purified
Purified ascites in 0.02 M Phosphate Buffered Saline, 0.25 M Sodium Chloride, 0.1% Sodium Azide,
pH 7.6 .
pH 7.6 .
Storage and Stability
Maintain at +2° to + 8°C for up to 12 months from date of receipt.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Daisuke Shimizu et al.
Japanese journal of ophthalmology, 62(6), 667-676 (2018-09-08)
Human cytomegalovirus (HCMV) infections can cause endotheliitis which is associated with an elevation in the intraocular pressure (IOP). However, the mechanism of the IOP elevation has not been established. The purpose of this study was to determine whether HCMV strains
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