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Merck
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MAB8051

Anti-Adenovirus Antibody, clone 2/6

clone 2/6, Chemicon®, from mouse

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
2/6, monoclonal
Species reactivity:
human
Application:
ELISA, FACS, IF, IHC
Citations:
6
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biological source

mouse

antibody form

purified immunoglobulin

clone

2/6, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, flow cytometry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable

isotype

IgG1κ

shipped in

wet ice

Quality Level

General description

Adenoviruses are 65-80 nm, non-enveloped, regular icosahedron pathogens often associated with respiratory and gastrointestinal illness as well as conjunctivitis. Well over 40 types of adenoviruses have currently been recognized. In addition to their deleterious effects, adenoviruses have been used in vaccine production and in gene therapy. They also have the ability to trigger cell proliferation by interfering with host cellular anti-oncogenes. Since they are easy to prepare, multiple quickly and efficiently, and have the ability to infect and deliver their genome (including researcher-created constructs) to the nucleus of a variety of post-mitotic cells, adenoviruses are extremely valuable in research and medical applications ranging from virus/host cell interaction to the insertion of novel genes or regulatory mechanisms into eukaryotic cellular systems, to delivery of therapeutic anti-cancer genes.

Application

Detect Adenovirus using this Anti-Adenovirus Antibody, clone 2/6 validated for use in ELISA, FC, IF, IH.
ELISA

IFA: acetone fixed cell preparations or tissues.

FACS: {McNees, 2004}

Dilute with buffer pH 7.5-8.0 to desired working volume.

For extensive dilution, protein containing or other stabilizing medium should be used.

Final working dilutions must be determined by end user.

Biochem/physiol Actions

All 41 serotypes of Adenovirus.

Physical form

Format: Purified
Protein A-purified immunoglobulin. Liquid at 1 mg/mL in 20 mM phosphate buffer, containing 25 mM NaCl and 0.1% sodium azide, pH 7.6

Preparation Note

Maintain at 2-8°C in convenient aliquots for up to 12 months. Do not store in a diluted format.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Modeling adenovirus latency in human lymphocyte cell lines.
Zhang, Y; Huang, W; Ornelles, DA; Gooding, LR
Journal of virology null
Postinternalization inhibition of adenovirus gene expression and infectious virus production in human T-cell lines.
McNees, AL; Mahr, JA; Ornelles, D; Gooding, LR
Journal of virology null
An outbreak of epidemic keratoconjunctivitis in a regional ophthalmology clinic in New South Wales.
K A Viney,P J Kehoe,B Doyle,V Sheppeard,A R Roberts-Witteveen,H Semirli,K A McPhie et al.
Epidemiology and Infection null
Protein crystals in Adenovirus type 5-infected cells: requirements for intranuclear crystallogenesis, structural and functional analysis.
Franqueville, L; Henning, P; Magnusson, M; Vigne, E; Schoehn, G; Blair-Zajdel, ME; Habib et al.
Testing null

Articles

呼吸道传染病由于其传染性强、传播速度快且发病率高,已成为我国重点防治的一类疾病。目前呼吸道病原体检测方法主要分为血清特异性抗体检测、抗原检测、多重核酸检测以及微生物检测系统。其中,相比于抗体检测,抗原检测在感染病毒初期、出现症状后即可做出检测,适用于疾病的早期诊断,对患者的治疗具有重要指导意义。

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