MAB5414
Anti-Notch 1 Antibody, extracellular, clone 8G10
culture supernatant, clone 8G10, Chemicon®
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Synonym(s):
Motch A, mT14, p300
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
hamster (Syrian)
Quality Level
antibody form
culture supernatant
antibody product type
primary antibodies
clone
8G10, monoclonal
species reactivity
mouse, rat
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... NOTCH1(4851)
Related Categories
General description
Notch 1 is a type 1 membrane protein that serves as a receptor for the membrane bound ligands jagged-1 and -2 and delta-1 to regulate cell fate determination through the implementation of differentiation, proliferation and apoptotic programs. Upon ligand binding, the Notch intracellular domain (NICD) is released by proteolytic processing and subsequently translocates to the nucleus where it forms a transcriptionally active complex with RBP-J kappa to activate genes of the enhancer of split locus. Notch-1 may also be involved in cell specification and/or differentiation and body segment determination in the postimplantation embryo. In the adult animal, Notch-1 is highly expressed in the brain, lung, and thymus with lower expression levels in the spleen, bone-marrow, spinal cord, eyes, mammary gland, liver, intestine, skeletal muscle, kidney and heart.
Specificity
Reacts with natural and recombinant Notch1. Recognizes a band migrating at approximately 300 kDa on Western blots of rat and mouse protein extracts. A cross reactive band migrating at approximately 230 kDa is observed in some samples and is believed to correspond to the processed extracellular fragment of Notch 1.
Immunogen
A GST fusion protein comprising amino acids 1299-1492 of mouse Notch 1
Epitope: extracellular
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Use Anti-Notch 1 Antibody, extracellular, clone 8G10 (Syrian Hamster Monoclonal Antibody) validated in FC, WB, IHC to detect Notch 1 also known as Motch A, mT14, p300.
Western blot
Immunohistochemistry on paraformaldehyde fixed frozen tissue sections.
Flow Cytometry
Optimal working dilutions must be determined by end user.
Immunohistochemistry on paraformaldehyde fixed frozen tissue sections.
Flow Cytometry
Optimal working dilutions must be determined by end user.
Linkage
Replaces: 04-1046
Physical form
Unpurified tissue culture supernatant from a perfusion system, filtered through a 0.2 micron membrane prior to vialing. Product contains 20% FBS and Ciprofloxacin at final concentration of 10 μg/mL.
Storage and Stability
Maintain at -20°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.
Analysis Note
Control
POSITIVE CONTROL: brain, lung, thymus.
POSITIVE CONTROL: brain, lung, thymus.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Paschalis Kratsios et al.
Circulation research, 106(3), 559-572 (2009-12-17)
The Notch signaling pathway is important for cell-cell communication that controls tissue formation and homeostasis during embryonic and adult life, but the precise cell targets of Notch signaling in the mammalian heart remain poorly defined. To investigate the functional role
Distinct TCR signaling pathways drive proliferation and cytokine production in T cells.
Guy, CS; Vignali, KM; Temirov, J; Bettini, ML; Overacre, AE; Smeltzer, M; Zhang, H; Huppa et al.
Nature Immunology null
Donna Nichol et al.
Blood, 116(26), 6133-6143 (2010-10-16)
Epidermal growth factor-like domain 7 (Egfl7) is important for regulating tubulogenesis in zebrafish, but its role in mammals remains unresolved. We show here that endothelial overexpression of Egfl7 in transgenic mice leads to partial lethality, hemorrhaging, and altered cardiac morphogenesis.
Sho Morioka et al.
The Journal of cell biology, 204(4), 607-623 (2014-02-19)
TNF activates three distinct intracellular signaling cascades leading to cell survival, caspase-8-mediated apoptosis, or receptor interacting protein kinase 3 (RIPK3)-dependent necrosis, also called necroptosis. Depending on the cellular context, one of these pathways is activated upon TNF challenge. When caspase-8
Changhui Ge et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(5), 1539-1544 (2008-01-30)
Mechanisms by which the extracellular domain of Notch1 controls Notch1 signaling are not well defined. Here, we show that the O-fucose glycan in the Notch1 ligand-binding domain regulates the strength of Notch1 signaling during embryogenesis, postweaning growth, and T cell
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