MAB5326C3
Anti-Nestin Antibody, clone 10C2, Cy3 conjugate
clone 10C2, from mouse, CY3 conjugate
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Synonym(s):
Nestin10c2, NES, Nbla00170, FLJ21841
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
mouse
Quality Level
conjugate
CY3 conjugate
antibody form
purified antibody
antibody product type
primary antibodies
clone
10C2, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... NES(10763)
General description
Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.
Specificity
This antibody recognizes human Nestin. Antibody is not reactive with rat or mouse Nestin protein.
Immunogen
Fusion protein corresponding to human Nestin.
Application
Detect Nestin using this Anti-Nestin Antibody, clone 10C2, Cy3 conjugate validated for use in ICC.
Research Category
Stem Cell Research
Neuroscience
Stem Cell Research
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism
Neuronal & Glial Markers
Neurofilament & Neuron Metabolism
Neuronal & Glial Markers
Quality
Evaluated by Immunocytochemistry in human RNcell CX neural stem cells (SCC007). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in human RNcell CX neural stem cells (SCC007).
Target description
The uncojugated parent antibody (Catalog No. MAB5326) has an observed MW at 200-220 kDa
Physical form
Protein A purified
Purified mouse monoclonal IgG1 conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.
Storage and Stability
Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.
Analysis Note
Control
RNcell CX neural stem cells (SCC007).
RNcell CX neural stem cells (SCC007).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Certificates of Analysis (COA)
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Teresa Coccini et al.
Current protocols, 2(4), e423-e423 (2022-04-27)
Neurotoxicity (NT) testing for regulatory purposes is based on in vivo animal testing. There is general consensus, however, about the need for the development of alternative methodologies to allow researchers to more rapidly and cost effectively screen large numbers of
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