MAB5290
Anti-Dopamine D1A Receptor Antibody, clone SG2-D1a
clone SG2-D1a, Chemicon®, from mouse
Sign Into View Organizational & Contract Pricing
All Photos(1)
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
SG2-D1a, monoclonal
species reactivity
mouse, sheep, rat
should not react with
pig, rabbit, human, bovine, canine
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2b
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... DRD1(1812)
Specificity
Dopamine Receptor D1A, no cross reactivity to D1B (D5) receptor. Immunoreactivity was found to be specific for mouse and rat D1A receptor. Human, pig, dog, rabbit, sheep and bovine samples were non-reactive by western analysis.
Immunogen
Recombinant rat D1a Receptor, carboxy terminus.
Application
Anti-Dopamine D1A Receptor Antibody, clone SG2-D1a detects level of Dopamine D1A Receptor & has been published & validated for use in IH & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
Western blot: 1:250-1:500 on unboiled membrane fractions from rat caudate tissue. Reactivity is also seen in perfrontal and temporal cortex. An identified band between 45-66kDa is observed (Luedtke, 1999).
Immunohistochemistry: 4% PFA fixed, vibratome sections, blocked with 3% NGS, 1% BSA with 0.2% triton X-100. Primary antibodies diluted onlyin PBS with excess blocking reagents.
Optimal working dilutions must be determined by the end user.
SUGGESTED WESTERN BLOT PROTOCOL
Solutions
10X Transfer buffer 1 liter
Tris 115.3 gm
Glycine 24.2 gm
[SDS 4 gm (may be omitted)]
For a 1X working solution: 1 liter
mix 700 mL water
200 mL MeOH
100 mL 10X TransferTBST
stock 1 liter
1M Tris pH 7.5 10 mL
5 M NaC 30 mL
10% Tween-20 5 mL
1. Run SDS-PAGE gel as desired.
2. Soak PVDF membrane (ImmobilonP from Millipore) in 100% methanol for 1-2 minutes to wet the membrane.
3. Soak membrane in transfer buffer (see below) until ready to set up blot transfer (>15 min).
4. Assemble gel-transfer sandwich with the blot toward the positive electrode.
5. Transfer protein from gel to membrane at 100 volts for 1-2 hours for small molecular weight proteins or 65 mA for 16 hours (overnight) for complete transfer of higher molecular weight proteins.
6. Stain the transferred bands with Chemicon BLOT-FastStain (Catalog Number 2076).
7. Destain with deionized water.
8. Block with 5% non-fat milk (Marvel or Carnation) in TBS or PBS, overnight at 4°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for 10 min, and filtered through glass filter (Gelman Acrodisc).
9. Incubation with first antibody overnight with gentle agitation at 4°C.
Optimal working dilutions and incubation time will need to be determined by the end user.
10. Wash at least 3 x 5 min. with TBST. From this stage, azide should be omitted.
11. Incubation with the secondary antibody (HRP-conjugated goat anti-mouse antibody, for example Chemicon Catalog Number AP124P, diluted appropriately) 1 h at room temperature.
12. Wash at least 3 x 5 min. with TBST.
13. Perform ECL with commercial kit (femtoLUCENT, Chemicon Catalog Number 2078).
Immunohistochemistry: 4% PFA fixed, vibratome sections, blocked with 3% NGS, 1% BSA with 0.2% triton X-100. Primary antibodies diluted onlyin PBS with excess blocking reagents.
Optimal working dilutions must be determined by the end user.
SUGGESTED WESTERN BLOT PROTOCOL
Solutions
10X Transfer buffer 1 liter
Tris 115.3 gm
Glycine 24.2 gm
[SDS 4 gm (may be omitted)]
For a 1X working solution: 1 liter
mix 700 mL water
200 mL MeOH
100 mL 10X TransferTBST
stock 1 liter
1M Tris pH 7.5 10 mL
5 M NaC 30 mL
10% Tween-20 5 mL
1. Run SDS-PAGE gel as desired.
2. Soak PVDF membrane (ImmobilonP from Millipore) in 100% methanol for 1-2 minutes to wet the membrane.
3. Soak membrane in transfer buffer (see below) until ready to set up blot transfer (>15 min).
4. Assemble gel-transfer sandwich with the blot toward the positive electrode.
5. Transfer protein from gel to membrane at 100 volts for 1-2 hours for small molecular weight proteins or 65 mA for 16 hours (overnight) for complete transfer of higher molecular weight proteins.
6. Stain the transferred bands with Chemicon BLOT-FastStain (Catalog Number 2076).
7. Destain with deionized water.
8. Block with 5% non-fat milk (Marvel or Carnation) in TBS or PBS, overnight at 4°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for 10 min, and filtered through glass filter (Gelman Acrodisc).
9. Incubation with first antibody overnight with gentle agitation at 4°C.
Optimal working dilutions and incubation time will need to be determined by the end user.
10. Wash at least 3 x 5 min. with TBST. From this stage, azide should be omitted.
11. Incubation with the secondary antibody (HRP-conjugated goat anti-mouse antibody, for example Chemicon Catalog Number AP124P, diluted appropriately) 1 h at room temperature.
12. Wash at least 3 x 5 min. with TBST.
13. Perform ECL with commercial kit (femtoLUCENT, Chemicon Catalog Number 2078).
Physical form
Format: Purified
Purified immunoglobulin. Liquid in 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
Storage and Stability
Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Carlo Cavallotti et al.
Neurochemical research, 29(8), 1499-1504 (2004-07-21)
Dopamine receptors (Dar) were studied as a component of the nervous dopaminergic system in the human dura mater. Dar were stained in several dural zones (vascular, perivascular, intervascular) in different regions (basal, calvarial, tentorial, occipital, frontal, parietal, temporal) of the
Anouk Schrantee et al.
PloS one, 12(2), e0172776-e0172776 (2017-02-28)
Dexamphetamine (AMPH) is a psychostimulant drug that is used both recreationally and as medication for attention deficit hyperactivity disorder. Preclinical studies have demonstrated that repeated exposure to AMPH can induce damage to nerve terminals of dopamine (DA) neurons. We here
Mizuki Maeda et al.
The Journal of veterinary medical science, 83(3), 542-548 (2021-02-02)
Recently, it has been reported that neonicotinoid pesticides (NNs) are transferred from mother to child and are assumed to affect the next generation, but the behavioral effects of NN exposure at different developmental stages have not been investigated. We exposed
Sufang Liu et al.
Molecular neurobiology, 57(10), 4060-4068 (2020-07-13)
Anterior cingulate cortex (ACC) is a critical brain center for chronic pain processing. Dopamine signaling in the brain has been demonstrated to contribute to descending pain modulation. However, the role of ACC dopamine receptors in chronic neuropathic pain remains unclear.
Naoki Yoneda et al.
The Journal of veterinary medical science, 80(4), 634-637 (2018-02-13)
Although neonicotinoid pesticides are expected to have harmful influence on mammals, there is little animal experimental data to support the effect and mechanisms. Since acetylcholine causes the release of dopamine, neonicotinoids may confer a risk of developmental disorders via a
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service