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MAB5276

Sigma-Aldrich

Anti-Endorphin β Antibody, clone 3-E7

clone 3-E7, Chemicon®, from mouse

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Synonym(s):
Anti-ACTH
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

3-E7, monoclonal

species reactivity

pig, human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable

isotype

IgG2a

NCBI accession no.

NM_000939.2
NM_001035256.1

UniProt accession no.

P01189

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... POMC(5443)

Specificity

The antibody reacts with the amino terminal H-Tyr-Gly-Gly-Phe sequence of human O-endorphin. Therefore, a high cross-reactivity with homologues of identical sequence, such as [Met]-enkephalin and [Leu]-enkephalin and a number of opioid peptides, occurs; the antibody also reacts with swine and camel O-endorphin. However, the antibody does not recognize human beta-lipotropin and N-acetyl-beta-endorphin (Gramsch et al., 1983; Meo et al., 1983; Herz et al., 1982).

Immunogen

Synthetic human beta-endorphin coupled with poly-lysine or thyroglobulin (Gramsch et al., 1983; Meo et al., 1983) (H-Tyr-Gly-Gly-Phe sequence).

Application

ELISA Immunohistochemistry

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neuroinflammation & Pain

Hormones & Receptors
This Anti-Endorphin Antibody, β, clone 3-E7 is validated for use in ELISA, IH for the detection of Endorphin.

Physical form

Format: Purified
Purified immunoglobulin. Liquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Fernanda B Lima et al.
Brain research, 1663, 1-8 (2017-03-13)
With the decline of ovarian steroids levels at menopause, many women experience an increase in anxiety and stress sensitivity. The locus coeruleus (LC), a central source of noradrenaline (NE), is activated by stress and is inhibited by β-endorphin. Moreover, increased
Rebecca J Gordon et al.
Journal of the Endocrine Society, 1(10), 1235-1246 (2017-12-22)
Hypothalamic proopiomelanocortin (POMC) is processed to α-melanocyte-stimulating hormone, which interacts with the melanocortin antagonist agouti-related protein (AgRP), to regulate energy balance. The POMC-derived opioid peptide β-endorphin (β-EP) also affects feeding behavior via interactions with brain µ-opioid receptors (MORs), including autoinhibitory
Miguel A Tejada et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(31), 8396-8401 (2017-07-19)
Sigma-1 antagonism potentiates the antinociceptive effects of opioid drugs, so sigma-1 receptors constitute a biological brake to opioid drug-induced analgesia. The pathophysiological role of this process is unknown. We aimed to investigate whether sigma-1 antagonism reduces inflammatory pain through the
Imam Subadi et al.
Iranian journal of medical sciences, 42(4), 384-391 (2017-08-02)
Wet cupping therapy is a complementary therapy in pain management. The mechanism of this therapy, however, needs further elucidation. Cells injured by wet cupping therapy seem to stimulate the expression of heat shock protein 70 (HSP70). Its benefit in pain
Gabrielle Page-Wilson et al.
American journal of physiology. Endocrinology and metabolism, 312(1), E19-E26 (2016-11-29)
The melanocortin neuronal system, which consists of hypothalamic proopiomelanocortin (POMC) and agouti-related protein (AgRP) neurons, is a leptin target that regulates energy balance and metabolism, but studies in humans are limited by a lack of reliable biomarkers to assess brain

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