Anti-Neurofilament 200kDa Antibody, clone NE14, Biotin Conjugate

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information and epitope maps, search "neurofilament" on our website.

Purified human neurofilament polypeptides (Debus et al., 1983).

Anti-Neurofilament 200kDa Antibody, clone NE14, Biotin Conjugate detects level of Neurofilament 200kDa & has been published & validated for use in ICC & IHC.

Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected Neurofilament in adult mouse brain cryosections.

Research Category
Neuroscience

Research Sub Category
Neurofilament & Neuron Metabolism

Evaluated by Immunocytochemistry in rat E18 primary cortex cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Neurofilament in rat E18 primary cortex cells.

Protein A purified

Purified mouse monoclonal IgG1 conjugated to Biotin in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Stable for 1 year at 2-8°C from date of receipt.

Control
Rat E18 primary cortex cells

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