MAB4051
Anti-Angiotensin Converting Enzyme Antibody, clone 9B9
clone 9B9, Chemicon®, from mouse
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All Photos(2)
Synonym(s):
ACE, CD143, DCP1, Peptidyl-dipeptidase A, Peptidase P
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
9B9, monoclonal
species reactivity
hamster, feline, human, monkey, rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
suitability
not suitable for immunohistochemistry (Paraffin)
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... AGT(183)
General description
The previously assigned protein identifier P22966 has been merged into P12821. Full details can be found on the UniProt database.
Specificity
Angiotensin-converting enzyme [ACE] (N-terminal), no reactivity to testicular/germinal form of human ACE (90kDa isoform)
Immunogen
Angiotensin-converting enzyme from human lung
Application
Anti-Angiotensin Converting Enzyme Antibody, clone 9B9 detects level of Angiotensin Converting Enzyme & has been published & validated for use in ELISA, IP, WB, IC, IH.
Immunohistochemistry (Danilov et al., 1987; Falkenhahn et al., 1995; Morrell et al., 1995) : 1:1,000 on fresh frozen or 4% paraformaldehyde fixed tissues. Does not work on paraffin embedded tissue.
Immunocytochemistry: 1:100. Reacts with endothelial cell, macrophages (activated) and THP1 cells (monocyte line).
Immunoblotting: 1:100: ~170-180kDa isoform, reducing conditions.
Immunoprecipitation: 1:1,000-1:10,000
ELISA : 1:1,000
Visualization of lung vessels (Danilov et al., 1989; Klibanov et al., 1988; Hiemisch et al., 1993)
Antibody mediated drug delivery into the lung (Danilov et al., 1991, 1994; Muzykantov et al., 1994, 1996)
Estimation of lung vessel injury (Muzykantov et al., 1991; Atochina et al., 1992)
Structure-function of ACE (Danilov et al., 1994)
Optimal working dilutions must be determined by the end user.
Immunocytochemistry: 1:100. Reacts with endothelial cell, macrophages (activated) and THP1 cells (monocyte line).
Immunoblotting: 1:100: ~170-180kDa isoform, reducing conditions.
Immunoprecipitation: 1:1,000-1:10,000
ELISA : 1:1,000
Visualization of lung vessels (Danilov et al., 1989; Klibanov et al., 1988; Hiemisch et al., 1993)
Antibody mediated drug delivery into the lung (Danilov et al., 1991, 1994; Muzykantov et al., 1994, 1996)
Estimation of lung vessel injury (Muzykantov et al., 1991; Atochina et al., 1992)
Structure-function of ACE (Danilov et al., 1994)
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Hormones & Receptors
Hormones & Receptors
Physical form
Format: Purified
Purified immunoglobulin. Liquid in PBS, pH 7.4, 150 mM NaCl containing 0.05% sodium azide.
Storage and Stability
Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Chiari Kojima et al.
The Journal of pharmacology and experimental therapeutics, 323(3), 855-860 (2007-09-20)
To elucidate an anti-inflammatory role of angiotensin-converting enzyme inhibitors (ACEIs) in cardiovascular disease, we studied the effect of ACEIs in monocyte adhesion to endothelial cells and underlying molecular mechanisms. Treatment of human monocytic THP-1 cells with monocyte chemoattractant protein-1 (MCP-1;
V V Petrov et al.
Journal of the renin-angiotensin-aldosterone system : JRAAS, 1(4), 342-352 (2002-04-23)
Appearance of angiotensin-converting enzyme (ACE) in fibrotic tissue can be the result of the action either of one particular growth factor or of cross-talk between multiple factors. Transforming growth factor-beta1 (TGF-beta(1)) is an effective inducor of the differentiation of cultured
Rosana I Reis et al.
American journal of physiology. Cell physiology, 315(3), C367-C379 (2018-06-07)
Hypertension is a global health problem, and angiotensin I (ANG I)-converting enzyme (ACE) inhibitors are largely used to control this pathology. Recently, it has been shown that ACE can also act as a transducer signal molecule when its inhibitors or
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