All Photos(1)
Synonym(s):
Anti-MYF4, Anti-bHLHc3, Anti-myf-4
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
monoclonal
species reactivity
rat, mouse, human
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... MYOG(4656)
Specificity
Myogenin. The epitope is localized to amino acids 158-178.
Immunogen
Recombinant protein from rat myogenin.
Application
Detect Myogenin with Anti-Myogenin Antibody (Mouse Monoclonal Antibody), that has been shown to work in IP, WB, ICC & IHC.
Research Category
Signaling
Signaling
Research Sub Category
Developmental Signaling
Developmental Signaling
Western blot: 1 μg/mL. Reacts with the ~34 kD protein.
Immunohistochemistry: 1-2 μg/mL on formalin-fixed, paraffin embedded human rhabdomyosarcoma tissue sections.
Immunocytochemistry
Immunoprecipitation: 2 μg/mg
Gel shift assays
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 1-2 μg/mL on formalin-fixed, paraffin embedded human rhabdomyosarcoma tissue sections.
Immunocytochemistry
Immunoprecipitation: 2 μg/mg
Gel shift assays
Optimal working dilutions must be determined by end user.
Physical form
Format: Purified
Purified immunoglobulin. Liquid in PBS containing 0.08% sodium azide.
Storage and Stability
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Rui Chen et al.
Frontiers in cell and developmental biology, 9, 671857-671857 (2021-09-08)
In the present study, the roles of a novel long non-coding RNA (lncRNA), lnc-GD2H, in promoting C2C12 myoblast proliferation and differentiation and muscle regeneration were investigated by quantitative polymerase chain reaction, western blotting, Cell Counting Kit-8, 5-ethynyl-2'-deoxyuridine (EdU), immunofluorescence staining
Sissel Beate Rønning et al.
Differentiation; research in biological diversity, 86(1-2), 13-22 (2013-08-13)
Primary muscle cell model systems from farm animals are widely used to acquire knowledge about muscle development, muscle pathologies, overweight issues and tissue regeneration. The morphological properties of a bovine primary muscle cell model system, in addition to cell proliferation
Renae J Stefanetti et al.
Frontiers in physiology, 5, 30-30 (2014-02-20)
Skeletal muscle atrophy is a critical component of the ageing process. Age-related muscle wasting is due to disrupted muscle protein turnover, a process mediated in part by the ubiquitin proteasome pathway (UPP). Additionally, older subjects have been observed to have
Peter Genovese et al.
Journal of tissue engineering and regenerative medicine, 15(12), 1131-1143 (2021-09-23)
Volumetric muscle loss (VML) is traumatic or surgical loss of skeletal muscle with resultant functional impairment. Skeletal muscle's innate capacity for regeneration is lost with VML due to a critical loss of stem cells, extracellular matrix, and neuromuscular junctions. Consequences
Yaping Nie et al.
Scientific reports, 7, 41608-41608 (2017-02-06)
Differentiation of myoblasts is essential in the development and regeneration of skeletal muscles to form multinucleated, contractile muscle fibers. However, the process of myoblast differentiation in mammals is complicated and requires to be further investigated. In this study, we found
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