Anti-Tau-1 Antibody, clone PC1C6, Alexa Fluor^™ 488 Conjugate

Tau, a microtubulebinding protein which serves to stabilize microtubules in growing axons, is found to be hyperphosphorylated in paired helical filaments (PHF), the major fibrous component of neurofibrillary lesions associated with Alzheimer’s disease. Hyperphosphorylation of Tau is thought to be the critical event leading to the assembly of PHF. Six Tau protein isoforms have been identified, all of which are phosphorylated by glycogen synthase kinase 3 (GSK 3). Cellular and subcellular localization: In situ, anti-tau-1 has a stringent specificity for the axons of neurons. The antibody does not stain the cell bodies or dendrites of neurons, nor does it stain any other cell type (4). However, this in vivo intracellular specificity is not maintained in culture: anti-tau-1 stains the axon, cell bodies, and dendrites of rat hippocampal neurons grown in culture (5). The specificity of anti-tau-1 was originally thought to represent the restricted expression of tau to axons. Later studies revealed that this specificity is dependant on the state of phosphorylation. In dephosphorylated samples (samples treated with alkaline phosphatase) anti-tau-1 stains astrocytes, perineuronal glial cells, and the axons, cell bodies and dendrites of neurons, while in untreated samples, anti-tau-1 stains only axons (6). (The epitope recognized by anti-tau-1 is probably at or near a phosphorylated site.)

This antibody binds to all known electrophoretic species of tau in human, rat, and bovine brain (one-dimensional SDS-PAGE).

Purified denatured bovine microtubule associated proteins.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

This Anti-Tau-1 Antibody, clone PC1C6, Alexa Fluor 488 Conjugate is validated for use in IC, IH for the detection of Tau-1.

Evaluated by Immunohistochemistry in adult mouse brain tissue.

Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected Tau-1 in adult mouse brain tissue.

79 kDa calculated. Uniprot describes isoforms ranging from 33 to 81 kDa

Protein A purified

Purified mouse monoclonal IgG1 conjugated to Biotin in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Control
Adult mouse brain tissue

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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