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MAB3418B

Sigma-Aldrich

Anti-MAP2 Antibody, clone AP20, Biotin Conjugated

clone AP20, from mouse, biotin conjugate

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Synonym(s):
Microtubule-associated protein 2, MAP-2, MAP2
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

biotin conjugate

antibody form

purified antibody

antibody product type

primary antibodies

clone

AP20, monoclonal

species reactivity

rat

species reactivity (predicted by homology)

bovine (immunogen homology), mouse (based on 100% sequence homology), chicken (based on 100% sequence homology), human (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable

isotype

IgG

UniProt accession no.

Q0IIA8

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

chicken ... Map2(424001)
human ... MAP2(4133)
mouse ... Map2(17756) , Map2(281294)
rat ... Map2(25595)

General description

The microtubule-associated protein 2 (MAP-2) is an abundant neuronal cytoskeletal protein that binds to tubulin, stabilizes microtubules (Herzog and Weber, 1978), and is an excellent marker for neurons. MAP-2 is essential for the development and maintenance of neuronal morphology (Matus, 1991). Three primary isoforms of MAP-2, the high molecular weight MAP2a and MAP2b, and the low molecular weight MAP2c (resulting from alternate splicing of the MAP2 gene), have been identified in neurours. (Chung et al., 1996). The low molecular weight isoform, MAP2c, is expressed in developing brain and is down-regulated during brain maturation, whereas the high molecular weight MAP2b is expressed in both developing and adult brain. The MAP2a appears only after brain maturation (Tucker, 1990). All these forms bind to microtubules through a domain near the carboxyl terminus that contains either three or four similar repeats of a 31-amino-acid motif (Lewis et al., 1988). MAP-2 together with MAP-4 and tau proteins belong to the family of thermostable proteins associated with microtubules.

Immunogen

Bovine brain microtubule protein

Application

Anti-MAP2 Antibody, clone AP20, Biotin Conjugated is an antibody against MAP2 for use in ICC.
Immunocytochemistry Analysis: A 1:25 dilution from a representative lot detected MAP2 in rat E18 primary cortex cells.

Quality

Evaluated by Immunohistochemistry in rat cortex tissue.

Immunohistochemistry Analysis: A 1:25 dilution of this antibody detected MAP2 in rat cortex tissue.

Target description

The unconjugated parent antibody (Cat. No. MAB3418) has an observed molecular weight at ~230 kDa

Physical form

Purified mouse monoclonal IgG conjugated to Biotin in PBS with 0.1% sodium azide and 15 mg/mL BSA.

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Scott D Bury et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 22(19), 8597-8606 (2002-09-28)
In humans and other animals, sufficient unilateral damage to the sensorimotor cortex can cause impairments in the opposite forelimb and the development of a hyper-reliance on the nonimpaired limb. This hyper-reliance is adaptive to the extent that it contributes to

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