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MAB331

Sigma-Aldrich

Anti-SNAP-25 Antibody, clone SP14

ascites fluid, clone SP14, Chemicon®

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UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

antibody form

ascites fluid

clone

SP14, monoclonal

species reactivity

pig, human, hamster, rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... SNAP25(6616)

Specificity

SNAP-25 (26-27 kDa). MAB331 is reactive with fusion protein from COS cells, but not with fusion protein from bacterial systems.

Immunogen

Crude synaptic immunoprecipitate (Human)

Application

Detect SNAP-25 using this Anti-SNAP-25 Antibody, clone SP14 validated for use in ELISA, WB, IH(P).
Immunohistochemistry at 1:500-1:5,000

Formalin fixed (<4 weeks), paraffin embedded

* It is suggested that you use an IgG specific second antibody to minimize possible non-specific staining.

Frozen

Western blotting at 1:100-1:1,000

ELISA at 1:500

It is reported that clone SP14 is capable of detecting SDS-resistant SNARE complexes {J. Cell Sci 2004 117(6):955-966}.

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Synapse & Synaptic Biology

Physical form

Liquid. Contains no preservative.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Matthew J Van Hook et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(17), 4618-4634 (2017-04-02)
G-protein βγ subunits (Gβγ) interact with presynaptic proteins and regulate neurotransmitter release downstream of Ca2+ influx. To accomplish their roles in sensory signaling, photoreceptor synapses use specialized presynaptic proteins that support neurotransmission at active zone structures known as ribbons. While
High sensitivity of mouse neuronal cells to tetanus toxin requires a GPI-anchored protein.
Munro, P; Kojima, H; Dupont, JL; Bossu, JL; Poulain, B; Boquet, P
Biochemical and biophysical research communications null
Toshihiko Shirafuji et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 38(2), 278-290 (2017-11-24)
Protein kinase Cγ (PKCγ) knock-out (KO) animals exhibit symptoms of Parkinson's disease (PD), including dopaminergic neuronal loss in the substantia nigra. However, the PKCγ substrates responsible for the survival of dopaminergic neurons in vivo have not yet been elucidated. Previously
The gene for soluble N-ethylmaleimide sensitive factor attachment protein alpha is mutated in hydrocephaly with hop gait (hyh) mice.
Hong, HK; Chakravarti, A; Takahashi, JS
Proceedings of the National Academy of Sciences of the USA null
Most peptide-containing sensory neurons lack proteins for exocytotic release and vesicular transport of glutamate.
Morris, JL; Konig, P; Shimizu, T; Jobling, P; Gibbins, IL
The Journal of Comparative Neurology null

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