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Key Documents

MAB3132

Sigma-Aldrich

Anti-Surfactant Protein D Antibody, lectin domain, clone IVF11

clone IVF11, Chemicon®, from mouse

Synonym(s):

SP-D

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

IVF11, monoclonal

species reactivity

human, rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NM_003019.4

UniProt accession no.

P35247

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... SFTPD(6441)

General description

Surfactant protein D is a Ca2+ dependent carbohydrate binding protein with a structure similar to other C-type mammalian lectins. Its molecular weight is 620 kDa under non-reducing conditions and 43 kDa under reducing conditions. The function of SP-D is not clearly defined. SP-D enhances the production of oxygen radicals in alveolar macrophages and regulates some actions of SP-A.

Specificity

MAB3132 recognizes an epitope localized in the lectin domain of rat surfactant protein D (SP-D). Reacts with alveolar type II cells, alveolar macrophages, types of Clara cells (in which SpD is highly concentrated), SP-A+ and SP-A-, and extracellular deposits. Alveolar type III cells are negative. No reaction on rat skin, colon, kidney or liver. SP-A cross reacitivity is typically evident at very low dilutions (1 to less than a few hundred) by ELISA.

Immunogen

Epitope: lectin domain
Purified rat surfactant protein D from bronchoalveolar lavage.

Application

Immunohistochemistry on snap frozen or paraffin embedded tissue.

Suggested working dilution for fresh rat tissue is 1:400 and for fresh human tissue is 1:20. Fixation: acetone 5 min at 4°C, paraformaldehyde, paraffin. Tissue pretreatment: microwave treatment 2 x 5 min at 750 W for paraffin tissues.

Western blot

ELISA: as capture antibody

Optimal working dilutions must be determined by the end user.
Research Category
Metabolism
Research Sub Category
Ion & Transport Channels
This Anti-Surfactant Protein D Antibody, lectin domain, clone IVF11 is validated for use in ELISA, IH(P), WB for the detection of Surfactant Protein D.

Linkage

Replaces: MAB3130

Physical form

Format: Purified
Immunoglobulin purified from tissue culture supernatant. Lyophilized from buffer containing 5 mg/mL BSA and 0.09% sodium azide.

Storage and Stability

Maintain lyophilized material at 2-8°C for up to 12 months from date of receipt. Reconstitute lyophilized material with 500 μL of sterile distilled water. After reconstitution, maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H312 + H332,H412

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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