MAB3101
Anti-Connexin 45 Antibody, near CT, cytoplasmic, clone 5B9.2
clone 5B9.2, Chemicon®, from mouse
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Synonym(s):
Cx45
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
5B9.2, monoclonal
species reactivity
rat, rabbit, human
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... GJC1(10052)
rat ... Gjc1(266706)
Specificity
Recognizes Human Connexin 45.
SPECIES REACTIVITIES:
Due to sequence homologies, this antibody is also expected to react with canine (100% homology) and mouse (92% homology) Connexin 45. Also confirmed to work on rat C6 cells. Others not tested.
SPECIES REACTIVITIES:
Due to sequence homologies, this antibody is also expected to react with canine (100% homology) and mouse (92% homology) Connexin 45. Also confirmed to work on rat C6 cells. Others not tested.
Immunogen
Epitope: near C-terminus, cytoplasmic
The peptide sequence used for the immunogen was H-Gln-Ala-Tyr-Ser-His-Gln-Asn-Asn-Pro-His-Gly-Pro-Arg-Glu-Cys-OH conjugated to KLH, corresponding to amino acids 354-367 of human connexin 45.
Application
Anti-Connexin 45 Antibody, near C-terminus, cytoplasmic, clone 5B9.2 detects level of Connexin 45 & has been published & validated for use in IC & WB.
Immunblotting (~46 kDa)
Immunocytochemistry
Optimal working dilutions must be determined by end user.
Immunocytochemistry
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
Physical form
Format: Purified
Liquid at 1 mg/mL in 0.02M PB with 0.25 M NaCl, pH=7.6 containing 0.1% sodium azide.
Storage and Stability
Maintain at 2-8°C in undiluted aliquots for up to 12 months.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Axonal synapses utilize multiple synaptic ribbons in the mammalian retina.
Kim, HL; Jeon, JH; Koo, TH; Lee, UY; Jeong, E; Chun, MH; Moon, JI; Massey, SC; Kim, IB
Testing null
J I Nagy et al.
The European journal of neuroscience, 46(2), 1790-1804 (2017-06-01)
Electrical synapses formed by connexin36 (Cx36)-containing gap junctions between interneurons in the cerebellar cortex have been well characterized, including those formed between basket cells and between Golgi cells, and there is gene reporter-based evidence for the expression of connexin45 (Cx45)
Richard Fischer et al.
Gastroenterology, 128(2), 433-448 (2005-02-03)
Gap junctional communication was studied in quiescent and activated hepatic stellate cells. Connexin expression and intercellular dye transfer were studied in rat hepatic stellate cells in culture and in vivo. Protein expression of connexin 43 was up-regulated in activated hepatic
Hideo Hoshi et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 29(28), 8875-8883 (2009-07-17)
The vertebrate retina is a distinctly laminar structure. Functionally, the inner plexiform layer, in which bipolar cells synapse onto amacrine and ganglion cells, is subdivided into two sublaminae. Cells that depolarize at light offset ramify in sublamina a; those that
Maria D Mayan et al.
The American journal of pathology, 182(4), 1337-1346 (2013-02-19)
Osteoarthritis (OA) is the most common joint disease and involves progressive degeneration of articular cartilage. The aim of this study was to investigate if chondrocytes from human articular cartilage express gap junction proteins called connexins (Cxs). We show that human
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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