Anti-Connexin 43 Antibody, clone 4E6.2

Connexins are highly homologous proteins that assemble as hexamers (connexons) to form gap junctions, small channels which allow for the passage of ions and other small molecules up to 1,200 daltons between neighboring cells. Gap junctions play an important role in a variety of cellular processes including homeostasis, intercellular metabolic cooperation and synchronization, morphogenesis, cell differentiation, and growth control. The most broadly studied connexins are the 26, 32, 43 and 45 kDa isoforms. Connexin 43 is a 382 a.a. protein belonging to the alpha-type (Group II) subfamily of connexin proteins and possessing four transmembrane regions, with cytoplasmic amino and carboxyl terminals. It undergoes rapid turnover in the cell and its monomers may reside in the ER/Golgi network, forming a reservoir available for assembly upon degradation of existing connexin-43 channels. Connexin 43 has been detected in a variety of tissues including: atrial and ventricular myocytes, granulose cells, astrocytes, fibroblasts, leptomeningeal cells, brain, lung, smooth muscle, uterine muscle, kidney, lens, epithelium, cornea and testis. Connexin 43 also may have a role in communication between migrating cardiac neural crest cells.

Connexin-43. Recognizes proteins of molecular weight 43-47 kDa on Western blots of mouse brain lysate. Mouse connexin 43 sequence is identical in rat and bovine, and differs by one amino acid in pig. This sequence has no known homology with other connexins. Also effective for detection of Cx43 in mouse keratinocyte cells and on frozen sections of rat heart.

A synthetic peptide corresponding to positions 252-270 of the mouse connexin 43 sequence. This sequence is identical in rat and bovine.

Epitope: C-terminus

Anti-Connexin 43 Antibody, clone 4E6.2 detects level of Connexin 43 & has been published & validated for use in ELISA, IC, IH & WB.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Western Blot (colorimetric detection):
1:1000-10,000 freshly prepared lysates, proteinase inhibitors are recommended. Recognizes proteins of molecular weight 43-47 kDa on Western blots of mouse brain lysate.

Immunocytochemistry:
A 1:50-1:250 dilution of a previous lot was used in immunocytochemistry.

Immunohistochemistry:
A previous lot was used to detect Connexin 43 expression in the keratinocytes of the middle and upper epidermal layers of frozen human skin tissue (Arita, Ken, et al. (2006). Am. J. Pathol. 169:416-423).

Immunohistochemistry:
A previous lot was used to detect Connexin 43 expression in human and rat heart tissue (1:500 dilution).

ELISA:
A previous lot of this antibody was used in ELISA.

Optimal working dilutions must be determined by end user.

Evaluated by Western Blot on rat brain lysates.

Western Blot Analysis:
1:1000 dilution of this antibody detected Connexin 43 on 10 μg of rat brain lysates

43-47 kDa

Format: Purified

Protein A purified

Purified mouse monoclonal IgG1 liquid in PBS containing sodium azide.

Stable for 1 year at 2-8ºC in undiluted aliquots from date of receipt.

Control
Mouse brain tissue lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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