MAB2137
Anti-Homing Receptor Antibody, clone A020
clone A020, Chemicon®, from rat
Synonym(s):
CD44s, HCAM, Pgp-1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rat
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
A020, monoclonal
species reactivity
mouse, human, rat, pig, rabbit, canine, bovine
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2b
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... CD44(960)
Related Categories
Specificity
Antibody reacts with the standard CD44 isoform (CD44s) that lacks exon sequences v1-v10. Also called human CD44 (85-95 kDa) gp90{Hermes} and mouse gp85 (Pgp-1).
Immunogen
Purified human blood lymphocyte CD44
Application
Anti-Homing Receptor Antibody, clone A020 is an antibody against Homing Receptor for use in ELISA, FC, IC, IH & WB.
ELISA 1:2000
Flow Cytometry :1:1000
Immunofluorescence 1:200
Immunohistostaining of paraffin embedded tissues. 1:250; antigen retrieval is usually not needed.
Immunoprecipitation 1:200
Western blotting: non-reduced conditions only; 90kDa band is detected; blocking PBS/5% milk protein, primary antibody dilution 1:1000 o/n incubation.
Optimal working dilutions must be determined by end user.
Flow Cytometry :1:1000
Immunofluorescence 1:200
Immunohistostaining of paraffin embedded tissues. 1:250; antigen retrieval is usually not needed.
Immunoprecipitation 1:200
Western blotting: non-reduced conditions only; 90kDa band is detected; blocking PBS/5% milk protein, primary antibody dilution 1:1000 o/n incubation.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
Target description
90 kDa
Physical form
Format: Purified
Liquid in 10 mM sodium phosphate, pH 7.5 with 150 mM NaCl and 0.01% sodium azide.
Protein A purified
Storage and Stability
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Cells of epithelium and highly expressed by carcinomas
Cells of epithelium and highly expressed by carcinomas
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Laura Novellasdemunt et al.
Stem cell reports, 18(2), 570-584 (2023-01-21)
Adenomatous polyposis coli (APC) mutation is the hallmark of colorectal cancer (CRC), resulting in constitutive WNT activation. Despite decades of research, targeting WNT signaling in cancer remains challenging due to its on-target toxicity. We have previously shown that the deubiquitinating
Sameerah Shaheen et al.
Stem cell reviews and reports, 12(4), 492-499 (2016-05-22)
Colorectal cancers (CRCs) form a disorganized hierarchy of heterogeneous cell populations on which current chemotherapy regimens fail to exert their distinctive cytotoxicity. A small sub-population of poorly differentiated cancer stem-like cells (CSCs), also known as cancer initiating cells, may exhibit
Carmela Ricciardelli et al.
BMC cancer, 13, 476-476 (2013-10-16)
Hyaluronan (HA) an important component of the extracellular matrix, has been linked to tumor progression and drug resistance in several malignancies. However, limited data is available for ovarian cancer. This study investigated the role of hyaluronan (HA) and a potential
Adam C Midgley et al.
The Journal of biological chemistry, 290(18), 11218-11234 (2015-02-27)
Hyaluronan (HA) promotes transforming growth factor (TGF)-β1-driven myofibroblast phenotype. However, HA can also have disease-limiting activity. Bone morphogenetic protein-7 (BMP7) is an antifibrotic cytokine that antagonizes TGF-β1, and isolated studies have demonstrated that HA can both mediate and modulate BMP7
Hsiao-Han Wang et al.
American journal of translational research, 9(11), 4785-4806 (2017-12-09)
Glioblastoma multiforme (GBM) is one of the most malignant and aggressive brain tumors with great amount of hyaluronan (HA) secretion and CD44 overexpression (HA receptor). CD44 has been suggested as a cancer stem cells (CSCs) marker. However, several clinical studies
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