Anti-Integrin β1 Antibody, activated, clone HUTS-4, Alexa Fluor^™ 555

Integrin beta-1 (UniProt P05556; also known as CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12, VLA-BETA, VLAB) gene (Gene ID 3688) in human. Integrin beta-1 is a 130 kDa transmembrane glycoprotein that interact with various integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptor complexes that bind to specific extracellular matrix proteins. Integrin receptors regulate a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor growth and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to the activation of signal transduction pathways and regulation of gene expression.

Clone HUTS-4 is specific for active conformation of human beta1 integrins. The beta1 or VLA integrin subgroup contains at least 10 members, each with a distinct alpha subunit nonconvalently associated with the common beta1 subunit. Functional activation of integrins can be experimentally induced by addition of Mg2+ or Mn2+, or by using one of several stimulatory antibodies that are available. Intracellular signals generated as a result of cell activation also modulate the confirmation and affinity of integrins in cellular systems. Antibodies such as clone HUTS-4 which have the ability to discriminate between different states of integrin activation are exceptionally useful for studies on the regulation of activation. Clone HUTS-4 recognizes epitopes in the 355-425 region of the common beta1 subunit of VLA integrins, whose expression parallels the ligand binding activity of these adhesion receptors induced by various extracellular and intracellular stimuli.

Epitope: A.a. 355-425 region of the common β1 subunit of VLA integrins.

Generated against human β1 integrins purified from Triton X-100 lysates of human lung, liver, and skeletal muscle tissues.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Integrin β1 in A431 cells.
The unconjugated antibody (Cat. No. MAB2079Z) is shown to be suitable also for Western blotting, ELISA, functional assay, and immunohistochemistry.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

This Anti-Integrin β1 Antibody, activated, clone HUTS-4, Alexa Fluor^™ 555 is validated for use in Immunocytochemistry for the detection of Integrin β1.

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Integrin β1 in HeLa cells.

~88 kDa observed.

Protein G purified

Purified mouse monoclonal IgG2a antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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