MAB1997
Anti-Integrin β1 Antibody, clone MB1.2
clone MB1.2, Chemicon®, from rat
Synonym(s):
CD29 antigen, Fibronectin receptor subunit beta, Integrin VLA-4 subunit beta, fibronectin receptor beta subunit, integrin VLA-4 beta subunit, integrin beta 1, integrin beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rat
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
MB1.2, monoclonal
species reactivity
mouse, human
species reactivity (predicted by homology)
rat
packaging
antibody small pack of 25 μg
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2κ
NCBI accession no.
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... ITGB1(3688)
Related Categories
General description
Integrin beta-1 (UniProt P05556; also known as CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12, VLA-BETA, VLAB) gene (Gene ID 3688) in human. Integrin beta-1 is a 130 kDa transmembrane glycoprotein that interact with various integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptor complexes that bind to specific extracellular matrix proteins. Integrin receptors regulate a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor growth and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to the activation of signal transduction pathways and regulation of gene expression.
Specificity
Reacts with β1 subunit of VLA (β1) integrins
Immunogen
Mouse Integrin beta-1, as per (Von Ballestrem, C.G., et al. (1996). Hybridoma. 15(2):125-132). Mouse Integrin beta-1, as per (Von Ballestrem, C.G., et al. (1996). Hybridoma. 15(2):125-132).
Application
Anti-Integrin β1 Antibody, clone MB1.2 is an antibody against Integrin β1 for use in FC, IH, IP & WB.
Immunoprecipitation:
A previous lot of this anitbody was used to immunoprecipitate VLA (β1) integrins from lysate of 106 cell equivalent: 2 μg.
Flow cytometry:
A 10 μg/mL concentration of a previous lot was used in FC.
Immunohistochemistry in frozen tissue sections:
20 μg/mL from a previous lot was used. As a result of the wide tissue distribution of VLA (β1), binding of MAB1997 to diverse cell types is commonly observed.
Does not block VLA integrin mediated cell adhesion to matrix proteins. Activation of VLA (β1) integrin signaling properties by MAB1997 has not been characterized.
Western blot:
10 µg/mL. Effective for samples that have been SDS-denatured and heated. Not effective for reduced samples.
Optimal working dilutions must be determined by end user.
A previous lot of this anitbody was used to immunoprecipitate VLA (β1) integrins from lysate of 106 cell equivalent: 2 μg.
Flow cytometry:
A 10 μg/mL concentration of a previous lot was used in FC.
Immunohistochemistry in frozen tissue sections:
20 μg/mL from a previous lot was used. As a result of the wide tissue distribution of VLA (β1), binding of MAB1997 to diverse cell types is commonly observed.
Does not block VLA integrin mediated cell adhesion to matrix proteins. Activation of VLA (β1) integrin signaling properties by MAB1997 has not been characterized.
Western blot:
10 µg/mL. Effective for samples that have been SDS-denatured and heated. Not effective for reduced samples.
Optimal working dilutions must be determined by end user.
Quality
Routinely evaluated by Western Blot on C2C12 lysate.
Western Blot Analysis:
1:1000 dilution of this lot detected integrin β1 on 10 μg of C2C12 lysate.
Western Blot Analysis:
1:1000 dilution of this lot detected integrin β1 on 10 μg of C2C12 lysate.
Target description
~88 kDa (non-glycosylated) and ~130 kDa ( glycosylated).
Linkage
Replaces: 04-1109
Physical form
Format: Purified
Purified rat monoclonal IgG2κ liquid in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl with 0.1% NaN3
Analysis Note
Control
Human OVCAR5 cell line whole cell lysate
C2C12 lysate.
Human OVCAR5 cell line whole cell lysate
C2C12 lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Not finding the right product?
Try our Product Selector Tool.
recommended
Product No.
Description
Pricing
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Won-Jing Wang et al.
The Journal of cell biology, 159(1), 169-179 (2002-10-09)
Death-associated protein kinase (DAP-kinase) is a calcium/calmodulin-dependent serine/threonine kinase, and participates in various apoptosis systems. However, its apoptosis-promoting mechanism is poorly understood. Here, we demonstrate that DAP-kinase suppresses integrin-mediated cell adhesion and signal transduction, whereas dominant-negative interference of this kinase
Reversion of the Jun-induced oncogenic phenotype by enhanced synthesis of sialosyllactosylceramide (GM3 ganglioside).
Miura, Y; Kainuma, M; Jiang, H; Velasco, H; Vogt, PK; Hakomori, S
Proceedings of the National Academy of Sciences of the USA null
?Np63 knockout mice reveal its indispensable role as a master regulator of epithelial development and differentiation.
Romano, RA; Smalley, K; Magraw, C; Serna, VA; Kurita, T; Raghavan, S; Sinha, S
Development null
Dystroglycan does not contribute significantly to kidney development or function, in health or after injury.
Jarad, G; Pippin, JW; Shankland, SJ; Kreidberg, JA; Miner, JH
American Journal of Physiology: Renal Physiology null
Olivier Destaing et al.
Molecular biology of the cell, 21(23), 4108-4119 (2010-10-12)
Invadosomes are adhesion structures involved in tissue invasion that are characterized by an intense actin polymerization-depolymerization associated with β1 and β3 integrins and coupled to extracellular matrix (ECM) degradation activity. We induced the formation of invadosomes by expressing the constitutive
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service