Anti-Integrin α5β1 Antibody

Integrin alpha-5 beta1 (UniProt: P08648/P05556); also known as CD49 antigen-like family member E, Fibronectin receptor subunit alpha/Fibronectin receptor subunit beta, VLA-5/ VLA-4 subunit beta, CD49e/CD29) is encoded by the ITGA5/ITGB1 (also known as FNRA/FNRB, MDF2, MSK12) gene (Gene ID: 3678/3688) in human. Integrins are heterodimeric integral membrane proteins composed of an a subunit and a b subunit that function in cell surface adhesion and signaling. Integrin a5 is expressed on thymocytes, T cells, monocytes, platelets, early B cells, and activated in B cells. Integrin a5 exhibits an increased expression in mesodermal progenitors and is shown to be prevalent in fetal cardiomyocytes. Its deletion is shown to cause mesodermal defects during embryogenesis in murine models. Membrane fractions obtained from fibroblasts of diabetic animals display significant reduction in integrin a5 levels. Integrin a5 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-41), which is subsequently cleaved off to generate the mature form that contains a heavy chain (aa 42-894) and a light chain (aa 895-1049). Structurally, it consists of an extracellular domain (aa 42-995), a transmembrane domain (aa 996-1021), and a cytoplasmic domain (aa 1022-1049). It contains four calcium binding sites (aa 480-488; 334-342; 401-409; and 465-473). When associated with b 1 subunit it can serve as a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. Integrin b 1 is synthesized with a signal peptide (aa 1-20) that is subsequently cleaved off. The mature form has an extracellular domain (aa 21-728), a helical domain (aa 729-751) and a cytoplasmic region (aa 752-798). (Ref.: Almeida, ME et al., (2016). Mol. Cell. Biochem. 421(1-2); 19-28; Neiman, G., et al. (2019). Sci. Rep. 9; 18077; Su, Y., et al. (2016). Proc. Natl. Acad. Sci. USA. 113(27); E3872-E3881).

Clone JBS5 is a mouse monoclonal antibody that detects human Integrin a5b1. It targets and epitope within the extracellular domain.

Jurkat cells.

Quality Control Testing

Evaluated by Flow Cytometry in HT-1080 cells.

Flow Cytometry Analysis: 1 μg of this antibody detected Integrin a5b1 in one million HT-1080 cells.

Tested Applications

ELISA Analysis: A representative lot detected Integrin a5 in ELISA applications (Mould, A.P., et. al. (1998). Biochem J. 331 ( Pt 3)(Pt 3):821-8).

Western Blotting Analysis: A representative lot detected Integrin a5 in Western Blotting applications (Ria, R., et. al. (2002). Haematologica. 87(8):836-45; Gu, C., et. al. (2001). Mol. Cell. Biol. 21(14):4579-97).

Immunocytochemistry Analysis: A representative lot detected Integrin a5 in Immunocytochemistry applications (Gong, X., et. al. (2018). EMBO J. 37(17):e98994).

Inhibition Analysis: A representative lot of this antibody produced inhibition of fibrinogen-, fibronectin- and laminin-mediated cell adhesion. (Wattam, B., et. al. (2001). Biochem J. 356(Pt 1):11-7; Rahman, S., et. al. (1998). Biochem J. 335 (Pt 2)(Pt2):247-57; Mould, A.P., et. al. (1998). Biochem J. 331 ( Pt 3)(Pt 3):821-8; Gu, C., et. al. (2001). Mol Cell Biol. 21(14):4579-97).

Immunohistochemistry Applications: A representative lot detected Integrin 5?1 in Immunohistochemistry applications (Hernandez, P.A., et. al. (2020). Sci Rep. 10(1):627; Qin, L., et. al. (2003). Biol Reprod. 69(2):563-71).

Immunoprecipitation Analysis: A representative lot detected Integrin 5?1 in Immunoprecipitation applications (Nishida, T., et. al. (2007). J Cell Commun Signal. 1(1):45-58; Gu, C., et. al. (2001). Mol Cell Biol. 21(14):4579-97).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Integrin a5β1, clone JBS5, Cat. No. MAB1969-I, is a mouse monoclonal antibody that detects Integrin a5b1 and is tested in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Inhibition Assay, and Western Blotting.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at +2°C to +8°C from date of receipt.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.