Anti-Integrin α5β1 Antibody, clone JBS5

Integrins are a family of dimeric, transmembrane proteins that mediate cell-cell and extracellular matrix adhesion. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. The integrin family is composed of at least 15 alpha and 8 beta subunits that may form over twenty different alpha-beta non-covalently bound dimeric combinations on the cell surface. The alpha subunits all have some homology to each other, as do the beta subunits. Both of the subunits contribute to the binding of the ligand. Integrin alpha subunits contain seven weak sequence repeats in the N-terminal region, which may be important in ligand binding, and have been predicted to fold cooperatively into a single beta-propeller domain with seven beta-sheets. The alpha-3 subunit (CD49c) is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. The alpha-3 subunit exists in two different splice variants, denoted as "A" and "B". The only difference that results from this differential splicing is a total change in the cytoplasmic domain, while the extracellular domain stays the same. Knock-out mice lacking this subunit show prenatal lethality and abnormalities in the kidneys.

Reacts with the human integrin alpha5beta1.

Jurkat Cells

Anti-Integrin α5β1 Antibody, clone JBS5 is an antibody against Integrin α5β1 for use in IH, IH(P) & IP.

Immunoprecipitation: 1-2 μg


Induction of homotypic aggregation: 100 pg/mL


Immunohistochemistry with paraformaldehyde fixation.


Paraffin embedded, PFA fixed tissue in monkey 1:50 with HIER {Qin et al., 2003}.


Inhibition of adhesion to fibronectin


Optimal dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Integrins

Ascites fluid containing no preservatives.

Unpurified

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Control
Mouse 3T3 fibroblasts Skin (Basement Membrane)

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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