MAB1940
Anti-Fibronectin Antibody, cellular, clone DH1
clone DH1, Chemicon®, from mouse
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Anti-CIG, Anti-ED-B, Anti-FINC, Anti-FN, Anti-FNZ, Anti-GFND, Anti-GFND2, Anti-LETS, Anti-MSF, Anti-SMDCF
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biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
DH1, monoclonal
species reactivity
rat, guinea pig, human, rabbit
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... FN1(2335)
rabbit ... Fn1(100328589)
rat ... Fn1(25661)
Specificity
Specific for the extra domain of cellular fibronectin (EDA sequence) and thus recognizes only the cellular fibronectin.
Immunogen
Epitope: cellular
Purified fibronectin from Human A8387 fibrosarcoma cells.
Application
Detect Fibronectin using this Anti-Fibronectin Antibody, cellular, clone DH1 validated for use in WB, IH.
Immunoblotting at 1:1,000
Indirect immunohistochemistry at 1:200-1:400
A 1-hour fixation with 3-3.5% paraformaldehyde in 0.1 M phosphate buffer pH 7.4 is suggested. Shows some reactivity on paraffin sections depending on fixation conditions and time (Latvala et al., 1996).
Final working dilutions must be determined by end user.
Indirect immunohistochemistry at 1:200-1:400
A 1-hour fixation with 3-3.5% paraformaldehyde in 0.1 M phosphate buffer pH 7.4 is suggested. Shows some reactivity on paraffin sections depending on fixation conditions and time (Latvala et al., 1996).
Final working dilutions must be determined by end user.
Physical form
Format: Purified
In PBS with 1% BSA and 0.1% sodium azide.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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