Anti-Neurofilament H & M Antibody, phosphorylated, clone NP1

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section.

Recognizes the KSP repeats of the phosphorylated forms of neurofilament-M (NF-M) and neurofilament-H (NF-H), because NF-L does contain a few of these Lysine-serine-PO4-proline repeats some cross reactivity to NF-L may be seen at very high antibody concentrations.

Bovine neurofilaments

Epitope: phosphorylated

Detect Neurofilament H & M using this Anti-Neurofilament H & M Antibody, phosphorylated, clone NP1 validated for use in ELISA, FC, RIA, WB, IC, IH(P).

Research Category
Neuroscience

Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers

Western blot : recognizes, 200kDa, and 150kDa NF-H and NF-M bands primarily. Staining is removed with phosphatase treatment. Because NF-L also contains lysine-serine PO4 sites, NP1 may cross react with NF-L as well, but this has not be substantiated in traditional westerns.

Immunohistochemistry. Works on paraffin embedded, formalin fixed and frozen acteone fixed tissue sections. 1:50-1:500 for immunofluorescence.

Immunocytochemistry on PC12, TERA-2 and other neuronal lines.

FACS ELISA/RIA

Optimal working dilutions must be determined by end user.

Replaces: 04-1061

Liquid. Contains no preservative.

Maintain at -20°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles.

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