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biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
TRYP11-236.7, monoclonal
mol wt
calculated mol wt 26.56 kDa
species reactivity
human
packaging
antibody small pack of 100 μg
technique(s)
ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
-10 to -25°C
General description
Trypsin-1 (UniProt: P07477; also known as EC:3.4.21.4, Beta-trypsin, Cationic trypsinogen, Serine protease 1, Trypsin I) is encoded by the PRSS1 (also known as TRP1, TRY1, TRYP1) gene (Gene ID: 5644) in human. Trypsin is pancreatic serine protease that is synthesized as an inactive precursor known as trypsinogen that is processed through the Golgi apparatus and packaged into zymogen granules for secretion by exocytosis. Trypsin-1 is synthesized with a signal peptide (aa 1-15) and a propeptide (trypsin activation peptide; aa 16-23) that are subsequently cleaved off to generate the mature form that can be further cleaved and processed into alpha-trypsin chain 1 (aa 24-122) and alpha-trypsin chain 2 (aa 123-247). Trypsin can be present in a single-chain and a two-chain form, produced by proteolytic cleavage after arginine 122. Trypsin can also undergo sulfation at tyrosine 154 that increases its selectivity towards basic versus apolar residues at the P2′ position of inhibitors that bind in a substrate-like fashion. Trypsin cleaves peptide chains mainly at the carboxyl side of the amino acid lysine or arginine, except when either is followed by proline.
Specificity
Clone TRYP11-236.7 is a mouse monoclonal antibody that detects human Trypsin-1.
Immunogen
Purified human pancreatic trypsin.
Application
Anti-Trypsin, human pancreas, clone TRYP11-236.7, Cat. No. MAB1482-I, is a mouse monoclonal antibody that detects Trypsin and is tested for use in and ELISA.
Immunohistochestry/IH(P): {Solakidi, et al. 2003} 1:100-1:500; formalin fixed, paraffin embedded human tissues; 5 m serial sections; blocking solution 0.5% serum in PBS; overnight incubations; enzymatic detection.
ELISA: (Dilution is assay based)
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
ELISA: (Dilution is assay based)
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Physical form
Purified mouse monoclonal in buffer containing PBS, pH 7.4, containing no preservatives without preservatives.
Storage and Stability
Maintain at -20°C in convenient aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Regulatory Information
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Certificates of Analysis (COA)
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