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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
CHYMO10-104.10, monoclonal
mol wt
calculated mol wt 28 kDa
species reactivity
human
packaging
antibody small pack of 100 μg
technique(s)
ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
-10 to -25°C
target post-translational modification
unmodified
Related Categories
General description
Chymotrypsin is a serine endopeptidase that is synthesized in the pancreatic acinar cells as chymotrypsinogen that is cleaved by trypsin to generate active chymotrypsin. Activation of chymotrypsinogen involves proteolytic cleavage at two sites. The resultant three chains are held together by five disulfide bonds. Chymotrypsin is reported to preferentially cleave peptide amide bonds where the carboxyl side of the amide bond (the P1 position) is a large hydrophobic amino acid (tyrosine, tryptophan, and phenylalanine). The aromatic ring in their sidechain that fits into a ′hydrophobic pocket′ (the S1 position) of the enzyme. Two predominant forms of chymotrypsin have been described and are designated as chymotrypsin A and B. They display high degree of homology but have significantly different proteolytic characteristics. The active site residues in chymotrypsin are far apart in the primary sequence but are brought together in a crevice formed between the two protein domains.
Specificity
Clone CHYMO10-104.10 is a mouse monoclonal antibody that detects human Chymotrypsin.
Immunogen
Purified human pancreatic chymotrypsin.
Application
Anti-Chymotrypsin (Hu Pancreas), clone CHYMO10-104.10, Cat. No. MAB1476-I, is a mouse monoclonal antibody that detects Trypsin and is tested for use in ELISA and Immunohistochemistry (Paraffin).
ELISA: (Dilution is assay dependent)
Immunohistochemistry: 1:1000.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Immunohistochemistry: 1:1000.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Physical form
Purified mouse monoclonal antibody in buffer containing PBS, pH 7.4, containing no preservatives.
Storage and Stability
Maintain at -20°C in convenient aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 L or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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