MAB1435
Anti-Macrophages/Monocytes Antibody, clone ED-1
clone ED-1, Chemicon®, from mouse
Synonym(s):
Anti-Monocyte Antibody, Clone ED-1 Anti-Macrophages, Monocytes Detection Antibody
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
ED-1, monoclonal
species reactivity
rat
should not react with
horse
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable
isotype
IgG1
shipped in
wet ice
target post-translational modification
unmodified
Related Categories
General description
Monocytes are produced by the bone marrow from haematopoietic stem cell precursors called monoblasts. Monocytes circulate in the bloodstream for about one to three days and then typically move into tissues throughout the body. They consist of between 3 to 8 percent of the leukocytes in the blood. In the tissues monocytes mature into different types of macrophages at different anatomical locations.
Macrophages are a type of white blood cell derived from monocytes that engulf invading antigenic molecules, viruses, and microorganisms and then display fragments of the antigen to activate helper T cells; ultimately stimulating the production of antibodies against the antigen.
Macrophages are a type of white blood cell derived from monocytes that engulf invading antigenic molecules, viruses, and microorganisms and then display fragments of the antigen to activate helper T cells; ultimately stimulating the production of antibodies against the antigen.
Specificity
Recognizes a single chain glycoprotein of 90-100 kD that is expressed on the lysomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. Studies have shown that the antigen has many characteristics in common with macrosialin in the mouse and CD68 in the human.
Clone ED1, which recognises a CD68 like molecule in rat is an excellent marker of activated microglia. For pan microglial markers, staining both resting and activated microglia, use like CD11b (MAB1387Z and others) or iba-1 (MABN92).
Clone ED1, which recognises a CD68 like molecule in rat is an excellent marker of activated microglia. For pan microglial markers, staining both resting and activated microglia, use like CD11b (MAB1387Z and others) or iba-1 (MABN92).
Immunogen
Rat spleen cells
Application
Immunohistology on frozen sections at 1:50-1:300. Paraffin embedded tissue (Bouin fixation): 1:50-1:300 (requires pretreatment with trypsin or pronase).
Flow cytometry at 1:100 - 1:200. Use 10 μL of this working dilution to label 10E6 cells.
Radioimmunoassay
Also suitable for Immunoprecipitation, and Western Blotting.
Optimal working dilutions must be determined by end user.
Flow cytometry at 1:100 - 1:200. Use 10 μL of this working dilution to label 10E6 cells.
Radioimmunoassay
Also suitable for Immunoprecipitation, and Western Blotting.
Optimal working dilutions must be determined by end user.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Immunoglobulins & Immunology
This Anti-Macrophages/Monocytes Antibody, clone ED-1 is validated for use in Flow Cytometry (FC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Radioimmunoassay (RIA), and Western Blotting (WB) for the detection of CD68.
Target description
90-100 kD
Linkage
Replaces: CBL1511; CBL260
Physical form
Format: Purified
Protein A purified.
Purified mouse IgG1 in PBS (pH 7.4) and 0.09% sodium azide
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Macrophages, monocytes, lymphoid organs
Macrophages, monocytes, lymphoid organs
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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