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Safety Information

MAB131873

Sigma-Aldrich

Anti-mCherry Antibody, clone 1C51

clone 1C51, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

1C51, monoclonal

species reactivity (predicted by homology)

all

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG2aκ

UniProt accession no.

X5DSL3

shipped in

ambient

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Anti-mCherry Antibody, clone 1C51 clone 1C51, from mouse

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Anti-MCAM/CD146 Antibody, clone 1C21 ZooMAb® Rabbit Monoclonal recombinant, expressed in HEK 293 cells

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Anti-MCAM/CD146 Antibody, clone 1C21 ZooMAb® Rabbit Monoclonal

clone

1C51, monoclonal

clone

5, monoclonal

clone

1C11, monoclonal, recombinant monoclonal

clone

1C21, recombinant monoclonal

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

shipped in

ambient

shipped in

-

shipped in

ambient

shipped in

ambient

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

technique(s)

affinity binding assay: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

General description

mCherry, red fluorescent protein, is derived from DsRed, a red fluorescent protein from disc corals of Discosoma species. It is monomeric form that has been engineered with multiple cycles of mutation, directed modification and evolutionary selection. It exhibits excitation maximum at 587 nm and an emission maximum at 610 nm with a fluorescent quantum yield of 0.22. mCherry displays high photostability and resistant to photobleaching. It also displays improved brightness and extremely rapid maturation rate. Anti-mCherry, clone1C51 is an affinity purified antibody that recognizes mCherry in HEK293 cells transfected with pFin-EF1-mCherry. (Ref.: Schaner, NC et al. (2004). Nat. Biotechnol. 22(12):1567-72).

Specificity

Clone 1C51 detects mCherry protein in multiple species.

Immunogen

His-tagged full length recombinant mCherry.

Application

Anti-mCherry Antibody, clone IC51, Cat. No. MAB131873 detects mCherry protein and is tested for use in Immunocytochemistry and Western Blotting.
Western Blotting Analysis: A representative lot detected mCherry in Western Blotting applications (Krajcovic, M., et. al. (2013). Mol Biol Cell. 24(23):3736-45).

Western Blotting Analysis: A representative lot detected mCherry in HEK293 cells transfected with pFin-EF1-mCherry vs HEK293 untransfected cells (Courtesy of Dr Gerry Shaw).

Immunocytochemistry Analysis: A representative lot detected mCherry in HEK293 cells transfected with pFin-EF1-mCherry (Courtesy of Dr Gerry Shaw).

Quality

Evaluated by Western Blotting in HEK293 cells transfected with pFin-EF1-mCherry.

Western Blotting Analysis: 1 µg/mL of this antibody detected mCherry in 10 µg of lysate from HEK293 cells transfected with pFin-EF1-mCherry.


Target description

~32 kDa Observed; 26.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified
Purified mouse monoclonal antibody IgG2a in buffer containing PBS with 0.03% sodium azide with 50% glycerol.

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Regulatory Information

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