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Key Documents

MAB1137Z

Sigma-Aldrich

Anti-Insulin Receptor Antibody, α subunit, azide free, clone 47-9

clone 47-9, Chemicon®, from mouse

Synonym(s):

CD220, MAB1137

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

47-9, monoclonal

species reactivity

human, pig, sheep, bovine

should not react with

rat, mouse

manufacturer/tradename

Chemicon®

isotype

IgG1

suitability

not suitable for Western blot
not suitable for immunoprecipitation

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... INSR(3643)

Specificity

Recognizes a protein of 135kDa, identified as the alpha-subunit of insulin receptor (IR). Its epitope localizes in exon 7/8 and shows no cross-reaction with IGF-receptors {Soos, 1986}. Antibody superbly inhibits (>90%) insulin binding and BLOCKS insulin action. IR is a heterotetrameric membrane glycoprotein consisting of disulfide-linked subunits in a beta-a-a-beta configuration. The beta-subunit (95kDa) possesses a single transmembrane domain, whereas the alpha-subunit is completely extracellular. The intracellular portion of the beta-subunit is a tyrosine-specific protein kinase with sequence homology to other such kinases. Activation of kinase is essential in signaling pathway whereby insulin regulates intracellular metabolism.

Immunogen

Epitope: alpha subunit, azide free
IM-9 lymphocytes followed by purified insulin receptor.

Application

Anti-Insulin Receptor Antibody, α subunit, azide free, clone 47-9 detects level of Insulin Receptor & has been published & validated.
Completely (>90%) Inhibits Insulin Binding: use antibody at 10nM

Antagonist: Blocks insulin action

Does not work in immunoprecipitation {Soos, 1986}.

Optimal working dilutions must be determined by end user.
Research Category
Signaling
Research Sub Category
Insulin/Energy Signaling

Physical form

Format: Purified
Protein G Purified
Protein G Purified mouse immunoglobulin in 10mM PBS, pH 7.4 contain no preservatives.

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
3T3 cells expressing transfected human insulin receptor

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Waldemar B Minich et al.
The Journal of clinical endocrinology and metabolism, 108(9), 2324-2329 (2023-03-05)
Severe insulin resistance (IR) in the presence of insulin receptor autoantibodies (InsR-aAb) is known as type B insulin resistance (TBIR). Considerable progress in therapy has been achieved, but diagnosis and monitoring of InsR-aAb remains a challenge. This work aimed to
Veronica Rivi et al.
Neuroendocrinology, 113(4), 406-422 (2022-10-13)
The Garcia effect, a solid learning paradigm, was used to investigate the molecular and behavioral effects induced by different lengths of fasting on the cognitive functions in the pond snail Lymnaea stagnalis, a valid model system. Three experimental groups were
Amer Youssef et al.
Endocrinology, 157(3), 1163-1174 (2016-01-14)
Placental mesenchymal stem cells (PMSCs) are readily available multipotent stem cells for potential use in regenerative therapies. For this purpose, PMSCs must be maintained in culture conditions that mimic the in vivo microenvironment. IGFs (IGF-1 and IGF-2) and oxygen tension
Carlos Godoy-Parejo et al.
Stem cells (Dayton, Ohio), 37(8), 1030-1041 (2019-04-26)
Insulin is present in most maintenance media for human embryonic stem cells (hESCs), but little is known about its essential role in the cell survival of individualized cells during passage. In this article, we show that insulin suppresses caspase cleavage

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