MAB1120
Anti-Insulin-like Growth Factor-1 Receptor Antibody, α-subunit, clone 24-31
clone 24-31, Chemicon®, from mouse
Synonym(s):
IGF-IR, CD221
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
24-31, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... IGF1R(3480)
Specificity
Recognizes the alpha-subunit of type I Insulin-like Growth Factor Receptor (IGF-1R). It shows no cross-reaction with insulin receptor. Its epitope localizes between a.a. 283-440 (exon 4-6) of IGF-1R. Both IGF-1R and insulin receptor are synthesized as a single polypeptide which is glycosylated and proteolytically cleaved to give the alpha- and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The a-subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity. It is assumed that, as for the insulin receptor, the tyrosine kinase activity is essential for IGF-1 action.
Weakly reacts with Rabbit.
Weakly reacts with Rabbit.
Immunogen
IGF-1R/3T3 mouse fibroblasts transfected with human type I IGF-receptor cDNA.
Application
Acts as a Weak IGF-like Agonist in Bioassays (Thymidine incorporation into DNA)
Immunoprecipitation (Use Protein G): 2μg antibody/mg protein lysate.
Immunohistochemistry (Frozen and formalin/paraffin): Use antibody at 2-4ug/mL for 30 min. at room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 min. followed by cooling at room temperature for 20 minutes. Please note that EDTA is better than citrate for epitope unmasking.
Optimal working dilutions must be determined by end user.
Immunoprecipitation (Use Protein G): 2μg antibody/mg protein lysate.
Immunohistochemistry (Frozen and formalin/paraffin): Use antibody at 2-4ug/mL for 30 min. at room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 min. followed by cooling at room temperature for 20 minutes. Please note that EDTA is better than citrate for epitope unmasking.
Optimal working dilutions must be determined by end user.
Anti-Insulin-like Growth Factor-1 Receptor Antibody, α-subunit, clone 24-31 detects level of Insulin-like Growth Factor-1 Receptor & has been published & validated for use in IH(P) & IP.
Research Category
Signaling
Signaling
Research Sub Category
Insulin/Energy Signaling
Insulin/Energy Signaling
Physical form
Format: Purified
Purified from ascites fluid by Protein G chromatography. Liquid in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
Storage and Stability
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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[Articles of the Hungarian Medical Journal from September, 2006].
Orvosi hetilap, 147(35-38), 1673-1864 (2006-09-03)
Phase I/II trial and pharmacokinetic study of cixutumumab in pediatric patients with refractory solid tumors and Ewing sarcoma: a report from the Children's Oncology Group.
Malempati, S; Weigel, B; Ingle, AM; Ahern, CH; Carroll, JM; Roberts, CT; Reid et al.
Journal of clinical oncology : official journal of the American Society of Clinical Oncology null
Becky K Brisson et al.
PloS one, 7(9), e45588-e45588 (2012-10-03)
Insulin-like growth factor-I (IGF-I) is an essential growth factor that regulates the processes necessary for cell proliferation, differentiation, and survival. The Igf1 gene encodes mature IGF-I and a carboxy-terminal extension called the E-peptide. In rodents, alternative splicing and post-translational processing
Amy M Smith et al.
Frontiers in cellular neuroscience, 16, 1047928-1047928 (2022-11-26)
The study of microglia isolated from adult human brain tissue provides unique insight into the physiology of these brain immune cells and their role in adult human brain disorders. Reports of microglia in post-mortem adult human brain tissue show regional
Y-F Huang et al.
British journal of cancer, 99(7), 1096-1102 (2008-09-11)
This study was aimed to identify the expression and the correlation of insulin-like growth factor-1 (IGF-1) system and their prognostic impacts in cervical cancer. Seventy-two patients with early-stage cervical cancer were eligible. We obtained the serum levels of total IGF-1
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