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Safety Information

MAB10758

Sigma-Aldrich

Anti-mouse Immunoglobulin kappa light chain Antibody, clone 187.1, Rat

clone 187.1, from rat

Synonym(s):

Kappa light chain

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

187.1, monoclonal

species reactivity

mouse

technique(s)

ELISA: suitable
flow cytometry: suitable

isotype

IgG1κ

shipped in

wet ice

Gene Information

mouse ... Igk(243469)

General description

Antibody molecules typically comprise two immunoglobulin light chains covalently bound to a pair of heavy chains. Immunoglobulin light chains occur in two types, designated by the Greek letters kappa and lambda. Kappa and lambda light chains are approximately 250 amino acids in length with an average mass of about 25 kDa. The ratio of kappa to lambda found in the immunoglobulin population varies by species.

Specificity

This antibody specifically recongizes the kappa light chain of mouse antibodies.

Immunogen

Epitope: Unknown
Mouse IgG2b, κ secreted by MPC-11 plasmacytoma

Application

Anti-mouse Immunoglobulin kappa light chain Antibody, clone 187.1, Rat is an antibody against mouse Immunoglobulin kappa light chain for use in ELISA, FC.
Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC. (Beck, K., et al. (2009). J. Exp. Med.206:2271-2284.)
Research Category
Secondary & Control Antibodies
Research Sub Category
Fragment Specific Secondary Antibodies

Quality

Evaluated by ELISA Analysis in a titer.

ELISA Analysis: This antibody was successfully used in a titer ELISA. Specificity of detection for mouse kappa light chain peptides is displayed below.

Target description

25 kDa Calculated

Physical form

Format: Purified
Protein G
Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10-13 - German Storage Class 10 to 13

Regulatory Information

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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