MAB1061
Anti-Bone Sialoprotein II Antibody, CT, clone ID1.2
clone ID1.2, Chemicon®, from mouse
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Synonym(s):
BSPII, Integrin-binding Sialoprotein
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
ID1.2, monoclonal
species reactivity
human
should not react with
bovine, rat, pig
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
radioimmunoassay: suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... IBSP(3381)
pig ... Ibsp(397137)
rat ... Ibsp(24477)
General description
Bone Sialoprotein II is one of the major noncollagenous proteins in the extracellular matrix of bone. It is a phosphorylated glycoprotein with an approximate molecular weight of 70 kDa. The protein has been found in osteoblasts and osteocytes. Diseases concerning bone turnover, pathological bone alterations, as well as the rapid increase of osteoporosis make it necessary to establish new markers. Bone Sialoprotein is discussed as a potential serum marker for monitoring bone remodeling.
Specificity
Recombinant and native human bone sialoprotein II (BSPII). Does not react with osteopontin (BSPI) or osteonectin.
Immunogen
Bone Sialoprotein isolated from human bones.
Epitope: C-terminus
Application
Research Category
Cell Structure
Cell Structure
Research Sub Category
Osteobiology
Osteobiology
This Anti-Bone Sialoprotein II Antibody, C-terminus, clone ID1.2 is validated for use in ELISA, RIA, WB, IC, IH(P) for the detection of Bone Sialoprotein II.
Western blot: (1μg/mL)
Immunohistochemistry on paraffin embedded tissue sections: (1μg/mL)
Immunocytochemistry: (1:100)
ELISA: (1μg/mL)
RIA
Optimal working dilutions must be determined by the end user.
Immunohistochemistry on paraffin embedded tissue sections: (1μg/mL)
Immunocytochemistry: (1:100)
ELISA: (1μg/mL)
RIA
Optimal working dilutions must be determined by the end user.
Linkage
Replaces: AB1855
Physical form
Format: Purified
Purified immunoglobulin from culture supernatant. Lyophilized from 50 mM TRIS, pH7.4.
Storage and Stability
Maintain lyophilized material at 2-8°C. Reconstitute with 100μL sterile distilled water and store at -20°C in undiluted aliquots up to 12 months. Avoid repeated freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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