MAB10005
Anti-ABCA1 Antibody, clone AB.H10
clone AB.H10, Chemicon®, from mouse
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ATP-binding cassette transporter 1, ABC-1, Cholesterol efflux regulatory protein
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biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
AB.H10, monoclonal
species reactivity
human, chicken, mouse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... ABCA1(19)
General description
Mutations in the ABCA1 gene are associated with Tangier disease (TD). TD is an autosomal recessive disorder results from an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and disorders in cellular lipid trafficking. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.
Specificity
Recognize specifically ABCA1 from human, mouse, and chicken.
Immunogen
Recombinant fragment, corresponding to amino acids 1800-2260 of Human ABCA1.
Application
ELISA
Immunohistochemistry (Paraffin): 5 μg/mL. Perform heat mediated antigen retrieval in sodium citrate buffer (pH 6), before commencing with IHC staining protocol. Amplification with biotin-streptavidin may be required.
Immunoprecipitation
Western Blot: 1 μg/mL. Predicted molecular weight: 254 kDa.
Optimal working dilutions must be determined by end user.
Immunohistochemistry (Paraffin): 5 μg/mL. Perform heat mediated antigen retrieval in sodium citrate buffer (pH 6), before commencing with IHC staining protocol. Amplification with biotin-streptavidin may be required.
Immunoprecipitation
Western Blot: 1 μg/mL. Predicted molecular weight: 254 kDa.
Optimal working dilutions must be determined by end user.
Research Category
Metabolism
Metabolism
Research Sub Category
Lipid Metabolism & Weight Regulation
Lipid Metabolism & Weight Regulation
This Anti-ABCA1 Antibody, clone AB.H10 is validated for use in ELISA, IP, WB, IH(P) for the detection of ABCA1.
Physical form
Format: Purified
Presented as a liquid in PBS, pH 7.4 with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4°C for 3 months.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Life science alliance, 5(9) (2022-05-17)
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