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M04S-03-5PK

Millipore

CellASIC ONIX switching plate mammalian cells (4 chamber)

The M04 switching plates enable continuous perfusion culture for live cell analysis. Laminar flow provides rapid & uniform solution exchange for maximum cell health. The glass coverslide bottom surface ensures the highest resolution optical viewing.

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UNSPSC Code:
41104923
eCl@ss:
32011202
NACRES:
NB.22

material

PMMA
glass
polycarbonate
polydimethylsiloxane (PDMS)

manufacturer/tradename

CellASIC® ONIX

W × L × H

85.48 mm × 127.76 mm × 14.35 mm

compatibility

for use with cells sized up to 50.0 μm

shipped in

ambient

Related Categories

General description

CellASIC® ONIX switching plate mammalian cells (4 chambers) enable continuous perfusion culture for live cell analysis. Laminar flow provides a rapid and uniform solution exchange for maximum cell health. The glass cover slide bottom surface ensures the highest resolution of optical viewing.

Application

CellASIC® ONIX switching plate mammalian cells(4 chambers) have been used:
  • in live-cell imaging to study the contribution of phi-particle in the autoinhibition of dynein and the activation of dynein by dynactin
  • in live-cell imaging of fcSAgA binding and immunoglobulin M surface clustering by fluorescence microscopy
  • in multiple epidermal growth factor (EGF)pulse experiments
The CellASIC® ONIX switching plate is suitable for use in:
  • time-lapse analysis of adherent cells
  • long-term continuous perfusion experiments (3 days typical)
  • solution exchange experiments (induction, inhibition, drug dosing, etc.)
  • automated immunostaining and on-demand fixation of live cells within the culture chamber
  • comparison of up to four different cell types or exposure conditions (media components) in parallel, temperature, and gas atmospheric control (temperature shift, anoxic conditions, etc.)

Features and Benefits

  • Compatible with CellASIC® ONIX2 Microfluidic system and ONIX2 Manifolds
  • Enables perfusion-based, long-term, live-cell analysis with solution switching
  • The plate offers a controlled and dynamic microenvironment for 2D and 3D cell cultures

Legal Information

CELLASIC is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Brittany L Hartwell et al.
Biomacromolecules, 18(6), 1893-1907 (2017-05-06)
A pressing need exists for antigen-specific immunotherapies (ASIT) that induce selective tolerance in autoimmune disease while avoiding deleterious global immunosuppression. Multivalent soluble antigen arrays (SAgAPLP:LABL), consisting of a hyaluronic acid (HA) linear polymer backbone cografted with multiple copies of autoantigen
Kai Zhang et al.
Cell, 169(7), 1303-1314 (2017-06-13)
Cytoplasmic dynein-1 binds dynactin and cargo adaptor proteins to form a transport machine capable of long-distance processive movement along microtubules. However, it is unclear why dynein-1 moves poorly on its own or how it is activated by dynactin. Here, we
Angel Stanoev et al.
Cell systems, 7(3), 295-309 (2018-08-27)
The proto-oncogenic epidermal growth factor receptor (EGFR) is a tyrosine kinase whose sensitivity to growth factors and signal duration determines cellular behavior. We resolve how EGFR's response to epidermal growth factor (EGF) originates from dynamically established recursive interactions with spatially

Articles

Dynamic Live Cell Imaging of Caspase Mediated Apoptosis and Cellular Hypoxia in Lymphocytes and Cancer Cells Using the CellASIC® ONIX2 Microfluidic Platform

Application note on how the CellASIC® ONIX2 microfluidic system can be used to analyze caspase-3 mediated apoptosis/cell death and cellular hypoxia in live immune and cancer cell lines.

Related Content

CellASIC® ONIX2 Microfluidic System

Convert your microscope to a live cell imaging system – the CellASIC® ONIX2 system allows precise manipulation of the cell culture environment for dynamic live cell imaging and analysis using mammalian, yeast, and bacterial cells.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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