PureProteome Magnetic Stand

Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.

PureProteome™ Protein A and G Magnetic beads provide a rapid and reproducible means to purify immunoglobulins (IgG) using the KingFisher Duo particle processor.

比较anti-FLAG® M2磁珠的小规模FLAG®标签蛋白纯化的不同洗脱方法。

通过 PureProteome™ Protein A 和 Protein G 磁珠，可利用 KingFisher Duo 磁珠纯化仪快速、可重现地纯化免疫球蛋白 (IgG)。

Immunoprecipitation (IP) is a powerful technique for proteomic screening, biomarker discovery, and signaling network elucidation. It is frequently used to enrich target proteins from complex samples such as cell lysates or extracts. Traditional IP protocols use Protein A, Protein G or a mixture of Protein A and G coupled to a solid support resin, such as agarose beads, to capture an antigen/antibody complex in solution. As the number of samples increase, the traditional, manual IP method can be time-consuming. Processing of multiple IP reactions in parallel can introduce complexity, variability and pipetting errors, which may affect reproducibility.

Biomarkers offer important information about homeostasis, disease, response to drug treatments, and environmental stimuli. Sera are rich sources of biomarkers (biological indicator proteins, peptides, small molecules, etc.) and are easier to sample than other tissues. However, the complexity of serum and the presence of highly abundant proteins like albumin and immunoglobulin can mask less abundant species, hindering biomarker detection. PureProteome albumin magnetic beads remove more than 98% of albumin from human serum. Here, we demonstrate that PureProteome albumin magnetic beads may also be used to remove albumin from mouse, guinea pig and rat sera. Depleted samples are often dilute, and may need concentration for downstream analyses. Therefore, we present a protocol for the convenient concentration of these samples using Amicon Ultra 2 mL centrifugal filters.