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IPFL20200

Millipore

Immobilon® -FL PVDF Membrane

10 sheets, 20cm x 20 cm, 0.45 µm pore size, transfer membrane with low background fluorescence

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Synonym(s):
Western blotting membrane, blotting membrane, transfer membrane
UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22

product name

Immobilon®-FL PVDF Membrane, 10 sheets, 20 cm x 20 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

material

PVDF membrane
plain filter
white filter

Quality Level

400

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable
western blot: suitable

filter L × W

20 cm × 20 cm

pore size

0.45 μm pore size

capacity

155 μg/cm2 adsorption capacity (insulin)
 205 μg/cm2 adsorption capacity (BSA)
 300 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black
for use with CPTS
for use with Coomassie brilliant blue
for use with Ponceau-S red

detection method

chemiluminescent
colorimetric
fluorometric

shipped in

ambient

Related Categories

General description

The Immobilon®-FL transfer membrane has a nominal pore size of 0.45 μm and is made up of polyvinylidene fluoride (PVDF) microporous membrane for binding proteins transferred from a variety of gel matrices. This membrane is hydrophobic and offers a uniformly controlled pore structure with a high binding capacity for biiomolecules. The membrane exhibits very low autofluorescence across a wide range of excitation/emission wavelengths. This property makes it ideal for blotting applications involving fluorescence-based immunodetection. In addition, the Immobilon®-FL membrane can be used for standard chemiluminescent or chromogenic detection. The Immobilon®-FL is recommended for optimal blotting of proteins with molecular weights greater than 20,000.

Application

Immobilon®-FL PVDF Membrane has been used in western blotting.

Features and Benefits

Immobilon®-FL Membrane:
  • Designed for optimal fluorescent immunodetection
  • Extremely low background improves the sensitivity of all fluorescence detection protocols
  • suitable for all commonly used fluorescent probes at all excitation and emission wavelengths
  • compatible with standard blocking agents and buffers
  • Ideal for multiplexing and chemifluorescence detection

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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J L Portis et al.
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Appropriate regulation of autophagy is crucial for clearing toxic proteins from cells. Defective autophagy results in accumulation of toxic protein aggregates that detrimentally affect cellular function and organismal survival. Here, we report that the microRNA miR-1 regulates the autophagy pathway

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