HNCSMAG-35K
MILLIPLEX® Human Circadian/Stress Magnetic Bead Panel - Multiplex Assay
About This Item
species reactivity
human
Quality Level
manufacturer/tradename
Milliplex®
assay range
accuracy: 129%
(Spike Recovery for Melatonin)
sensitivity: 4.3 pg/mL
(Melatonin; MinDC+2SD)
sensitivity: 686 pg/mL
(Cortisol; MinDC+2SD)
standard curve range: 3.4-2,500 pg/mL
(Melatonin)
standard curve range: 686-500,000 pg/mL
(Cortisol)
inter-assay cv: <25%
intra-assay cv: <15%
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex xMAP)
shipped in
wet ice
Related Categories
General description
The MILLIPLEX® Human Circadian/Stress Bead Panel is to be used for the simultaneous quantification of Cortisol and Melatonin. This is a configurable assay, and either or both analytes can be selected. This kit may be used for the analysis of human serum, plasma, tissue/cell lysate, culture supernatant samples, or cerebrospinal fluid (CSF). Serum or plasma samples should be extracted using either the acetonitrile precipitation technique or the Waters 96-well HLB extraction plate. Please refer to the protocol for more information on both methods. The necessary components to perform the extractions are not included with this kit.
Panel Type: Neuroscience
Specificity
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Application
- Analytes: Cortisol, Melatonin
- Recommended Sample type: serum, plasma, cerebrospinal fluid (CSF), tissue/cell lysate, and culture supernatant samples
- NOTE: Sample extraction protocols are required for serum/plasma samples. Other sample types (e.g. CSF, culture supernatant, saliva, etc.) do not require sample extraction.
- Recommended Sample dilution: 1:4 for extracted serum and plasma or neat for CSF and culture supernatant
- Assay Run Time: Overnight
- Research Category: Neuroscience Metabolism
- Research Subcategory: Category Metabolic Disorders Obesity Inflammation & Autoimmune Mechanisms
- NOTE: This is a competitive assay
Features and Benefits
Packaging
Components
Human Circadian/Stress Quality Control 1, 1 vial, lyophilized
Human Circadian/Stress Quality Control 2, 1 vial, lyophilized
Assay Buffer, 2 bottles, 30 mL ea
Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
Human Circadian/Stress Primary Antibodies, 1 bottle, 3.5 mL
Human Circadian/Stress Detection Antibodies, 1 bottle, 5.5 mL
Human Circadian/Stress Antibody-Immobilized Magnetic Beads, 20X, 200 uL ea
Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL
Mixing Bottle, 1 bottle
Sample Plate, 96 well format, 1 plate with 2 Foil Plate Sealers
Storage and Stability
Legal Information
Disclaimer
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1
Storage Class Code
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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