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HDIAB-34K-PMX5

Millipore

MILLIPLEX® Human Diabetes Panel, Premixed 5 Plex Assay

The Human Diabetes, Premixed 5 Plex Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 5 protein biomarkers in human serum, plasma and cell culture samples.

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000

species reactivity

human

manufacturer/tradename

Milliplex®

assay range

accuracy: 86%
(GLP-1 (Active))
accuracy: 91%
(Insulin)
accuracy: 92%
(C-Peptide)
accuracy: 94%
(Leptin)
sensitivity: 15.8 pg/mL
(MinDC+2SD; Glucagon)
sensitivity: 16.4 pg/mL
(MinDC+2SD; C-Peptide)
sensitivity: 2.1 pg/mL
(MinDC+2SD; GLP-1 (Active))
sensitivity: 32.7 pg/mL
(MinDC+2SD; Insulin)
sensitivity: 65.9 pg/mL
(MinDC+2SD; Leptin)
standard curve range: 13.7-10,000 pg/mL
(Glucagon)
standard curve range: 137-100,000 pg/mL
(Insulin)
standard curve range: 137-100,000 pg/mL
(Leptin)
standard curve range: 2.7-2,000 pg/mL
(GLP-1 (active))
standard curve range: 27.4-20,000 pg/mL
(C-Peptide)
inter-assay cv: <15%
intra-assay cv: <10%
(C-Peptide)
inter-assay cv: <15%
intra-assay cv: <10%
(Glucagon)
inter-assay cv: <15%
intra-assay cv: <10%
(Insulin)
inter-assay cv: <15%
intra-assay cv: <10%
(Leptin)
inter-assay cv: <20%
intra-assay cv: <10%
(GLP-1 (Active))

technique(s)

multiplexing: suitable

compatibility

configured for Premixed

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

General description

Type I Diabetes is an early onset autoimmune disease that destroys insulin producing pancreatic β-cells. Type II Diabetes is later onset metabolic disorder caused by obesity, poor diet and a sedentary lifestyle. With insufficient levels of insulin, or resistance to insulin, elevated glucose can lead to heart disease, stroke, kidney failure and dementia.

MILLIPLEX® Human Diabetes Premixed Bead Panel is a 5-plex kit to be used for the simultaneous quantification of the following analytes in serum, plasma, or culture supernatant samples: C-Peptide, GLP-1 (Active), Glucagon, Insulin, Leptin.

This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Metabolism

Specificity

Cross Reactivty
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

  • Analytes: C-Peptide, Glucagon-like Peptide-1 (GLP-1) (Active), Glucagon, Insulin, Leptin
  • Recommended Sample Type: Human serum, plasma, tissue/cell culture supernatants and lysates
  • Recommended Sample Dilution: 25 μL per well of undiluted serum or plasma; tissue/cell culture samples may require a dilution with an appropriate control medium.
  • NOTE: Protease inhibitors must be added to blood upon collection, DPPIV inhibitor for GLP-1 and Aprotinin for Glucagon. See protocol for details.
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C
  • Research Category: Metabolism
  • Research Subcategory: Obesity, Diabetes, Diabetes - Type 1, Diabetes - Type 2

Packaging

Everything you need in a single kit.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Please contact Technical Service for linearity of dilution.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Skull and crossbonesEnvironment
GHS06,GHS09

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Regulatory Information

新产品

Certificates of Analysis (COA)

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Sergejs Berdnikovs et al.
The Journal of allergy and clinical immunology, 149(5), 1802-1806 (2021-11-07)
The roles of systemic and airway-specific epithelial energy metabolism in altering the developmental programming of airway epithelial cells (AECs) in early life are poorly understood. Our aim was to assess carbohydrate metabolism in developing AECs among children with and without

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