About This Item
Product Name
MILLIPLEX® Human Circulating Cancer Biomarker Magnetic Bead Panel - Cancer Multiplex Assay, Circulating Cancer Biomarker Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple oncology biomarkers in various tumor types in human serum, plasma and cell culture samples.
species reactivity
human
manufacturer/tradename
Milliplex®
assay range
accuracy: 35-108%
standard curve range: 0.09-67 mU/mL
(β-HCG)
standard curve range: 0.7-500 pg/mL
(IL-6)
standard curve range: 1.3-1000 pg/mL
(IL-8, TNFα)
standard curve range: 2.7-2000 pg/mL
(TRAIL, TGF-α)
standard curve range: 6.9-5000 pg/mL
(SCF)
technique(s)
multiplexing: suitable
Quality Level
detection method
fluorometric (Luminex® xMAP® technology)
shipped in
wet ice
Legal Information
Other Notes
Human Circulating Cancer Biomarker Panel 1 Quality Controls 1 and 2 (HCC-6058)
Serum Matrix (HCP-SM)
Note: Contains 0.08% Sodium Azide
Set of one 96-Well Plate with 2 Sealers (MAG-PLATE)
Assay Buffer (L-AB)
Note: Contains 0.08% Sodium Azide
10X Wash Buffer (L-WB)
Note: Contains 0.05% Proclin
Human Circulating Cancer Biomarker Panel 1 Detection Antibodies (HCC-1058 or HCC-1058-2)
Streptavidin-Phycoerythrin (L-SAPE 4)
Bead Diluent (LBD-4)
Application
- Analytes: α-Fetoprotein (AFP), CA125, CA15-3, CA19-9, CEA, CYFRA21-1, sFas (TNFRSF6), sFasL, FGF-2 (FGF-basic), β-HCG (HCGβ), HE4, HGF, IL-6, IL-8 (CXCL8), Leptin, MIF, Osteopontin (OPN), Prolactin, PSA (free)*, PSA (total)*, SCF, TGFα, TNFα, TRAIL (TNFSF10), VEGF.
- Note: Total PSA and Free PSA cannot be plexed together
- Recommended Sample Type: Human serum, plasma or tissue/cell culture supernatants or lysates
- Recommended Sample Dilution: 25 μL per well of 1:6 diluted plasma or serum, or 25 μL cell culture psaesaa sample undiluted or diluted in appropriate control medium
- Assay Run Time: Overnight (16-18 hr) at 2-8°C
- Research Category: Cancer
Biochem/physiol Actions
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Disclaimer
Features and Benefits
General description
MILLIPLEX® Circulating Cancer Biomarker Panel 1 is a 25-plex kit that can be used for the simultaneous quantification of any or all of the following analytes in serum, plasma and tissue/cell lysate and culture supernatant samples: AFP, CA125, CA15-3, CA19-9, CEA, CYFRA21-1, sFas, sFasL, FGF2, β-HCG, HE4, HGF, IL-6, IL-8, Leptin, MIF, Osteopontin, Prolactin, PSA (free), PSA (total), SCF, TGFα, TNFα, TRAIL and VEGF. Note that certain biomarkers are tumor specific, such as PSA, while others, such as IL-8, have been detected in many cancers. PSA (free) and PSA (total) cannot be plexed together. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Circulating Cancer
Packaging
Preparation Note
signalword
Danger
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
target_organs
Respiratory Tract
Storage Class
10 - Combustible liquids
wgk
WGK 3
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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