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HANG2MAG-12K

Millipore

MILLIPLEX® Human Angiogenesis Panel 2, HANG2MAG-12K

Angiogenesis Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple angiogenic biomarkers in human serum, plasma and cell culture samples.

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84

Quality Level

200

species reactivity

human

manufacturer/tradename

Milliplex®

assay range

accuracy: 88-97%
standard curve range: 137-100,000 pg/mL
(Thrombospondin-2)
standard curve range: 137-100,000 pg/mL
(sVEGFR2)
standard curve range: 137-100,000 pg/mL
(suPAR)
standard curve range: 14-10,000 pg/mL
(sAXL)
standard curve range: 14-10,000 pg/mL
(sHER3)
standard curve range: 14-10,000 pg/mL
(sVEGFR1)
standard curve range: 27-20,000 pg/mL
(sIL-6Rα)
standard curve range: 27-20,000 pg/mL
(sPECAM-1)
standard curve range: 27-20,000 pg/mL
(sTIE-2)
standard curve range: 274-200,000 pg/mL
(Angiostatin)
standard curve range: 274-200,000 pg/mL
(sE-Selectin)
standard curve range: 274-200,000 pg/mL
(sNeuropilin-1)
standard curve range: 41-30,000 pg/mL
(Osteopontin (OPN))
standard curve range: 41-30,000 pg/mL
(sEGFR)
standard curve range: 41-30,000 pg/mL
(sHER2)
standard curve range: 41-30,000 pg/mL
(sc-Kit/sSCFR)
standard curve range: 412-300,000 pg/mL
(sVEGFR3)
standard curve range: 69-50,000 pg/mL
(sHGFR)
standard curve range: 7-5,000 pg/mL
(PDGF-AB/BB)

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

Related Categories

General description

Angiogenesis, the development of new vascular networks, is a key process in normal growth and development as well as in wound healing, with homeostasis maintained by a delicate balance of angiogenic factors and inhibitors. Consequently, insufficient, or excessive blood vessel growth underlies many diseases, including cardiovascular disease, diabetic ulcers, macular degeneration, and cancer. Angiogenesis plays a significant role in tumor growth and metastasis.

MILLIPLEX® Human Angiogenesis Magnetic Bead Panel 2 is a 19-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, cell culture samples, and tissue homogenates/lysates: Angiostatin, soluble E-Selectin, (sE-Selectin), Osteopontin (OPN), Platelet Derived Growth Factor-AB/BB (PDGF-AB/BB), soluble Platelet Endothelial Cell Adhesion Molecule-1 (sPECAM-1), Thrombospondin-2 (TSP-2), soluble AXL (sAXL), soluble c-Kit/Stem Cell Growth Factor Receptor (sc-Kit/SCFR), soluble Epidermal Growth Factor Receptor (sEGFR)†, soluble Human Epidermal Growth Factor Receptor 2 (sHer2), soluble Human Epidermal Growth Factor Receptor 3 (sHer3), soluble Hepatocyte Growth Factor Receptor/c-Met (sHGFR/c-Met), soluble IL-6Rα, soluble Neuropilin-1 (sNRP-1), soluble Tie-2 (sTie-2), soluble Urokinase-type Plasminogen Activator Receptor (suPAR), soluble Vascular Endothelial Growth Factor Receptor 1 (sVEGFR1), soluble Vascular Endothelial Growth Factor Receptor 2 (sVEGFR2), and soluble Vascular Endothelial Growth Factor Receptor 3 (sVEGFR3). This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

†Antibody against active site results in preferential binding to unbound EGFR.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Circulating Cancer

Application

  • Analytes: Angiostatin, soluble AXL (sAXL) , soluble c-Kit/Stem Cell Growth Factor Receptor (sc-Kit/SCFR), soluble E-Selectin (sE-Selectin), soluble Epidermal Growth Factor Receptor (sEGFR/sErbB1/sHER1)†, soluble Human Epidermal Growth Factor Receptor 2 (sHer2/sHER2/sErbB2), soluble Human Epidermal Growth Factor Receptor 3 (sHer3/ sHER3 / sErbB3), soluble Hepatocyte Growth Factor Receptor (sHGFR/c-Met/sc-Met), soluble IL-6Rα (IL-6Rα (soluble)/sIL-6Rα), soluble Neuropilin-1 (sNRP-1), Osteopontin (OPN), Platelet Derived Growth Factor-AB/BB (PDGF-AB/BB), soluble Platelet Endothelial Cell Adhesion Molecule-1 (sPECAM-1/sCD31), Thrombospondin-2 (TSP-2), soluble Tie-2 (sTie-2), soluble Urokinase-type Plasminogen Activator Receptor (suPAR), soluble Vascular Endothelial Growth Factor Receptor 1 (soluble VEGFR1, sVEGFR1/ sFlt-1), soluble Vascular Endothelial Growth Factor Receptor 2 (soluble VEGFR2/sVEGFR2/ sKDR/sFlk-1), soluble Vascular Endothelial Growth Factor Receptor 3 (soluble VEGFR3/sVEGFR3/ sFlt-4).
  • †Antibody against active site results in preferential binding to unbound EGFR.
  • Recommended Sample Type: Human serum, plasma, cell culture supernatants and tissue/cell homogenates/lysates
  • Recommended Sample Dilution: 25 μL per well of 1:5 diluted plasma or serum; cell culture supernatant may be used undiluted or diluted with appropriate control medium
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C
  • Research Category: Cancer

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Other Notes

Please contact Technical Service for linearity of dilution.
Sensitivity: See kit protocol for individual analytes sensitivities.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Regulatory Information

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Sayuri Padayachee et al.
Nigerian journal of physiological sciences : official publication of the Physiological Society of Nigeria, 37(1), 29-34 (2022-08-11)
A dysregulation of angiogenic mediators has been implicated in HIV infection. Inconsistent data exists on highly active antiretroviral therapy (HAART) usage in pregnancy and its association with PE development. In view of the high prevalence of HIV infection and PE
Nicos Mitsides et al.
Nephron, 143(4), 234-242 (2019-09-13)
Progressive chronic kidney disease (CKD) inevitably leads to salt and water retention and disturbances in the macro-and microcirculation. We hypothesize that salt and water dysregulation in advanced CKD may be linked to inflammation and microvascular injury pathways. We studied 23
Juan Pablo Hinestrosa et al.
Communications medicine, 2, 29-29 (2022-05-24)
Detecting cancer at early stages significantly increases patient survival rates. Because lethal solid tumors often produce few symptoms before progressing to advanced, metastatic disease, diagnosis frequently occurs when surgical resection is no longer curative. One promising approach to detect early-stage
Luka Roškar et al.
Journal of clinical medicine, 10(4) (2021-03-07)
Preoperative determination of the extent of endometrial cancer (EC) would avoid the complications associated with radical surgery. Screening of patients' plasma biomarkers might enable a more precise diagnosis of EC and a tailored treatment approach. This prospective case-control monocentric pilot

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