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HADK1MAG-61K

Millipore

MILLIPLEX® Human Adipokine Magnetic Bead Panel 1 - Endocrine Multiplex Assay

The Human Adipokine Panel 1, using the Luminex xMAP technology, enables the simultaneous analysis of five biomarkers in human serum or plasma samples.

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47

description

Refer to protocol

Quality Level

200

species reactivity

human

manufacturer/tradename

Milliplex®

assay range

accuracy: 87%
(Resistin)
accuracy: 89%
(Adiponectin)
sensitivity: 10 pg/mL
(MinDC+2SD; Adipsin)
sensitivity: 21 pg/mL
(MinDC+2SD; Adiponectin)
sensitivity: 3.5 pg/mL
(MinDC+2SD; Lipocalin-2/NGAL)
sensitivity: 4.4 pg/mL
(MinDC+2SD; Resistin)
sensitivity: 5.8 pg/mL
(MinDC+2SD; PAI-1)
standard curve range: 13-200,000 pg/mL
(Adipsin)
standard curve range: 26-400,000 pg/mL
(Adiponectin)
standard curve range: 3.2-50,000 pg/mL
(Lipocalin-2/NGAL)
standard curve range: 6.4-100,000 pg/mL
(Resistin)
standard curve range: 9.6-150,000 pg/mL
(PAI-1 (total))
inter-assay cv: <10%
intra-assay cv: <10%
(Adipsin)
inter-assay cv: <10%
intra-assay cv: <15%
(Adiponectin)
inter-assay cv: <10%
intra-assay cv: <15%
(Lipocalin-2/NGAL)
inter-assay cv: <10%
intra-assay cv: <20%
(PAI-1 (total))
inter-assay cv: <10%
intra-assay cv: <20%
(Resistin)

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

Related Categories

General description

The role of white adipose tissue was once thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete several hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism. The Adipokine Adiponectin is involved in the regulation of lipid and glucose metabolism, influencing the body′s response to insulin. Adiponectin also has anti-inflammatory effects on the cellular lining of blood vessel walls which may help explain the association of high levels with reduced risk of heart attack.

The MILLIPLEX® Human Adipokine Panel 1 is a 5-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum or plasma samples: Adiponectin, Adipsin, Lipocalin-2/NGAL, Total PAI-1, and Resistin. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Metabolism

Application

  • Analytes: Adiponectin Adipsin Lipocalin-2/NGAL PAI-1 (Total) Resistin
  • Recommended Sample Type: Human serum and plasma
  • Recommended Sample Dilution: 25 μL per well of 1:400 diluted serum or plasma
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C
  • Research Category: Metabolism
  • Research Subcategory: Endocrine

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Other Notes

Please contact Technical Service for linearity of dilution.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Pictograms

Skull and crossbonesEnvironment
GHS06,GHS09

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Regulatory Information

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Ida Heir Hovland et al.
European journal of nutrition, 59(3), 1013-1029 (2019-04-12)
To examine whether supplementation with low doses of fish or milk proteins would affect glucose regulation and circulating lipid concentrations in overweight healthy adults. Ninety-three overweight adults were assigned to receive 2.5 g protein/day from herring (HER), salmon (SAL), cod (COD)
Diana Silva et al.
PloS one, 14(4), e0215475-e0215475 (2019-04-19)
Adipose tissue-derived adipokines are pro-inflammatory cytokines involved in metabolic-related diseases and can be influenced by diet and exercise. We aimed to compare the effect of a Mediterranean (MdM) compared with Fast Food (FFM) meal on the exercise induced adipokines changes.
A pilot study of active vitamin D administration and insulin resistance in African American patients undergoing chronic hemodialysis.
Hung, AM; Sundell, MB; Plotnikova, NE; Bian, A; Shintani, A; Ellis, CD; Siew, ED; Ikizler, TA
Journal of Renal Nutrition : the Official Journal of the Council on Renal Nutrition of the National Kidney Foundation null
Vinicius J B Martins et al.
The British journal of nutrition, 112(6), 937-944 (2014-07-30)
Undernutrition in early life has been reported to be closely associated with the development of non-communicable diseases in adulthood. Adequate treatment is important for reversing these effects. In the present study, we investigated the effects of undernutrition and anthropometric recovery
Emily Eruysal et al.
Alzheimer's & dementia (Amsterdam, Netherlands), 11, 646-653 (2019-09-14)
Lipocalin-2 is an acute-phase protein with pleotropic functions that has been implicated in several diseases including Alzheimer's disease (AD). However, it is unknown if circulating lipocalin-2 levels are altered in the preclinical stage of AD, where AD pathology has accumulated
View All Related Papers

Related Content

Multiplexing Metabolic Syndrome Biomarkers | MILLIPLEX® Assays

Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service