Skip to Content
Merck
CN
All Photos(3)

Documents

Safety Information

HADCYMAG-61K

Millipore

MILLIPLEX® Human Adipocyte Magnetic Bead Panel - Endocrine Multiplex Assay

The Human Adipocyte Magnetic Bead Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 11 Adipocyte protein biomarkers in cell culture samples.

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84

Quality Level

200

species reactivity

human

manufacturer/tradename

Milliplex®

assay range

accuracy: 100%
(IL-6)
accuracy: 102%
(TNFα)
accuracy: 103%
(MCP-1)
accuracy: 83%
(PAI-1 (total))
accuracy: 99%
(HGF)
sensitivity: 0.2 pg/mL
(MinDC+2SD; IL-8)
sensitivity: 0.2 pg/mL
(MinDC+2SD; NGF)
sensitivity: 0.2 pg/mL
(MinDC+2SD; TNF?)
sensitivity: 0.6 pg/mL
(MinDC+2SD; IL-6)
sensitivity: 10 pg/mL
(MinDC+2SD; Leptin)
sensitivity: 15 pg/mL
(MinDC+2SD; Adiponectin)
sensitivity: 2.0 pg/mL
(MinDC+2SD; IL-1?)
sensitivity: 2.1 pg/mL
(MinDC+2SD; MCP-1)
sensitivity: 3.0 pg/mL
(MinDC+2SD; HGF)
sensitivity: 4.4 pg/mL
(MinDC+2SD; Resistin)
sensitivity: 4.6 pg/mL
(MinDC+2SD; PAI-1 (total))
standard curve range: 0.29-4,000 pg/mL
(IL-8)
standard curve range: 0.32-5,000 pg/mL
(NGF)
standard curve range: 0.38-6,000 pg/mL
(TNFα)
standard curve range: 0.51-8,000 pg/mL
(IL-1β)
standard curve range: 0.96-150,000 pg/mL
(IL-6)
standard curve range: 0.96-150,000 pg/mL
(MCP-1)
standard curve range: 26-400,000 pg/mL
(Adipnectin)
standard curve range: 6.4-100,000 pg/mL
(HGF)
standard curve range: 6.4-100,000 pg/mL
(Resistin)
standard curve range: 9.6-150,000 pg/mL
(Leptin)
standard curve range: 9.6-150,000 pg/mL
(PAI-1)
inter-assay cv: <15%
intra-assay cv: <10%
(Adiponectin)
inter-assay cv: <15%
intra-assay cv: <10%
(IL-8)
inter-assay cv: <15%
intra-assay cv: <10%
(MCP-1)
inter-assay cv: <15%
intra-assay cv: <10%
(Resistin)
inter-assay cv: <20%
intra-assay cv: <10%
(HGF)
inter-assay cv: <20%
intra-assay cv: <10%
(IL-6)
inter-assay cv: <20%
intra-assay cv: <10%
(NGF)
inter-assay cv: <20%
intra-assay cv: <10%
(PAI-1 (total))
inter-assay cv: <20%
intra-assay cv: <10%
(TNFα)
inter-assay cv: <20%
intra-assay cv: <15%
(IL-1β)
inter-assay cv: <25%
intra-assay cv: <20%
(Leptin)

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

Related Categories

General description

The role of white adipose tissue was once thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete several hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism. One of these Adipokines, Adiponectin, is involved in the regulation of lipid and glucose metabolism, influencing the body′s response to insulin. Adiponectin also has anti-inflammatory effects on the cellular lining of blood vessel walls which may help explain the association of high levels with reduced risk of heart attack.

The MILLIPLEX® Human Adipocyte Panel is an 11-plex kit to be used for the simultaneous quantification of any or all of the following analytes in cell/tissue culture samples: Adiponectin, HGF, IL-β, IL-6, IL-8, Leptin, MCP-1, NGF, PAI-1 (total), Resistin, TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Metabolism

Specificity

Cross Reactivty
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

  • Analytes: Adiponectin, HGF, IL-1β, IL-6, IL-8, Leptin, MCP-1, NGF, PAI-1 (Total), Resistin, TNF-α
  • Recommended Sample Type: Tissue/cell culture supernatants or extracts
  • Recommended Sample Dilution: 25 μL per well of undiluted tissue/cell culture sample; samples may require dilution with an appropriate medium prior to assay
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C
  • Research Category: Metabolism
  • Research Subcategory: Endocrine

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

96 well plate
Everything you need in a single kit.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Please contact Technical Service for linearity of dilution.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Skull and crossbonesEnvironment
GHS06,GHS09

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Skin Sens. 1

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Regulatory Information

新产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Tracey McLaughlin et al.
Journal of the American Heart Association, 10(22), e021003-e021003 (2021-11-03)
Background Inflammation in epicardial adipose tissue (EAT) may contribute to coronary atherosclerosis. Myocardial bridge is a congenital anomaly in which the left anterior descending coronary artery takes a "tunneled" course under a bridge of myocardium: while atherosclerosis develops in the
Katharina Kessler et al.
Nutrients, 12(2) (2020-02-06)
: Meal timing affects metabolic regulation in humans. Most studies use blood samples for their investigations. Saliva, although easily available and non-invasive, seems to be rarely used for chrononutritional studies. In this pilot study, we tested if saliva samples could
Jelizaveta Sokolovska et al.
Physiological reports, 8(13), e14506-e14506 (2020-07-12)
Interval walking training has demonstrated more pronounced positive effects on physical fitness and metabolism in type 2 diabetes (T2D), compared to continuous walking. One of the pathogenic mechanisms of T2D is associated with derangements in leptin/adiponectin axis, which might predispose
Sonja Suvakov et al.
EBioMedicine, 70, 103536-103536 (2021-08-15)
Preeclampsia is a pregnancy-specific hypertensive disorder characterized by proteinuria and/or multisystem involvement. Disease-specific therapy has yet to be developed due to the lack of understanding of underlying mechanism(s). We postulate that accelerated ageing in general, and particularly cellular senescence, play
Pilar Codoñer-Franch et al.
Clinical biochemistry, 47(1-2), 44-48 (2013-11-05)
To evaluate whether serum resistin levels are related to cardiovascular risk in obese children. Cross-sectional study of 110 children (40 normal weight and 70 severely obese). Clinical and biochemical parameters, including lipid profile, fasting glucose and insulin, and homocysteine, were
View All Related Papers

Related Content

Multiplexing Metabolic Syndrome Biomarkers | MILLIPLEX® Assays

Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service